Stern R, Wilczek J, Thorpe W P, Rosenberg S A, Cannon G
Cancer Res. 1980 Feb;40(2):325-8.
Cells derived from osteogenic sarcomas and from Ewing's sarcomas, two malignant bone tumors, were examined for the types of collagens they elaborated into the tissue culture media. Type I procollagen was the predominant species from all osteogenic sarcoma cell lines, a finding consistent with bone cell origin. The Ewing's sarcoma cells contained a prominent peak of type III procollagen and resembled the profile of vascular smooth muscle cells. Fibroblasts derived from skin biopsies taken from amputation specimens synthesized both type I and type III procollagens at the expected ratio of approximately 3:1. The examination of matrix proteins may provide a general classification scheme for human sarcomas and permit distinction of one tumor from another, as well as from normal fibroblasts.
对源自骨肉瘤和尤因肉瘤这两种恶性骨肿瘤的细胞进行了检测，以确定它们分泌到组织培养基中的胶原蛋白类型。I型前胶原蛋白是所有骨肉瘤细胞系中的主要类型，这一发现与骨细胞起源一致。尤因肉瘤细胞含有一个突出的III型前胶原蛋白峰，其特征与血管平滑肌细胞相似。取自截肢标本皮肤活检的成纤维细胞以约3:1的预期比例合成I型和III型前胶原蛋白。对基质蛋白的检测可能为人类肉瘤提供一个通用的分类方案，并有助于区分一种肿瘤与另一种肿瘤，以及与正常成纤维细胞的区别。
