Bainton D F, Maloney M A, Patt H M, Stern R
J Exp Med. 1986 Feb 1;163(2):400-13. doi: 10.1084/jem.163.2.400.
Rabbit stromal fibroblasts subcultured from red and yellow bone marrow and implanted beneath the renal capsule form ossicles the hemic cellularity of which mirrors the cellularity of the marrow used for culture. Although the cultured red and yellow marrow cells are similar in fine-structural appearance, they differ strikingly in enzymatic content of alpha-naphthylbutyrate esterase, which is abundant only in the cells derived from yellow marrow. Other observers (20, 21) have proposed that stromal fibroblasts are preadipocytes, and this data suggests that those derived from yellow marrow have the phenotype of more differentiated adipocytes. On the other hand, fibroblasts derived from red and yellow bone marrow show no differences in their profiles of procollagen synthesis. Both types of fibroblasts secrete type III procollagen as the major species, with a I/III ratio of 1:3; in contrast, rabbit dermal fibroblasts have a prominent peak of type I procollagen. The similarity of stromal cells derived from red and yellow bone marrow in procollagen synthesis suggests that the collagen part of the extracellular matrix is not the only basis for their intrinsic difference in capacity for hematopoiesis.
从红骨髓和黄骨髓中传代培养的兔基质成纤维细胞，植入肾被膜下后形成小骨，其半细胞结构反映了用于培养的骨髓的细胞结构。虽然培养的红骨髓和黄骨髓细胞在精细结构外观上相似，但它们在α-萘丁酸酯酶的酶含量上有显著差异，该酶仅在源自黄骨髓的细胞中丰富。其他研究者（20, 21）提出基质成纤维细胞是前脂肪细胞，这些数据表明源自黄骨髓的细胞具有更分化的脂肪细胞表型。另一方面，源自红骨髓和黄骨髓的成纤维细胞在原胶原合成谱上没有差异。两种类型的成纤维细胞都以III型原胶原作为主要分泌产物，I/III比例为1:3；相比之下，兔真皮成纤维细胞有一个突出的I型原胶原峰。源自红骨髓和黄骨髓的基质细胞在原胶原合成上的相似性表明，细胞外基质的胶原部分不是它们造血能力内在差异的唯一基础。
