Tomecki K J, Montague P M, Hearing V J
J Natl Cancer Inst. 1980 Jan;64(1):29-32.
We used gel electrophoresis to fractionate serum and urine samples of patients with malignant melanoma and of healthy volunteers (controls) and observed that a) all proteins found in the sera of melanoma patients also occurred in the sera of 2 or more healthy volunteers, b) two minor protein bands were present in the sera of 73% of the melanoma patients but in the sera of only 38% of the controls, and c) one major band was present in the sera of 73% of the melanoma patients but in the sera of only 12% of the controls. We characterized these proteins by molecular size and charge. Two proteins, analogous in electrophoretic behavior to those found more frequently in melanoma patients' sera, were observed exclusively in the urine from melanoma patients. The demonstration of such differences in serum and urine proteins warrants the further study of this system as a serologic and/or immunologic test for diagnosis of malignant melanoma.
我们采用凝胶电泳法对恶性黑色素瘤患者及健康志愿者（对照组）的血清和尿液样本进行分离，并观察到：a）在黑色素瘤患者血清中发现的所有蛋白质也存在于两名或更多健康志愿者的血清中；b）73%的黑色素瘤患者血清中有两条次要蛋白带，而对照组血清中仅有38%有此情况；c）73%的黑色素瘤患者血清中有一条主要蛋白带，而对照组血清中仅有12%有此情况。我们通过分子大小和电荷对这些蛋白质进行了表征。在黑色素瘤患者尿液中仅观察到两种蛋白质，其电泳行为与在黑色素瘤患者血清中更频繁发现的蛋白质类似。血清和尿液蛋白质中存在此类差异，这使得对该系统作为恶性黑色素瘤诊断的血清学和/或免疫学检测进行进一步研究成为必要。
