Twentyman P R, Brown J M, Gray J W, Franko A J, Scoles M A, Kallman R F
J Natl Cancer Inst. 1980 Mar;64(3):595-604.
A new tumor model system (RIF-1) was developed that is very suitable for studies in which clonogenic survival is compared with growth delay and control probability following various forms of treatment. The tumor was a radiation-induced sarcoma in the inbred female C3H/Km mouse. It had a low median tumor dose, had a satisfactory plating efficiency direct from in vivo to in vitro, was nonimmunogenic or minimally immunogenic, and metastasized only at a relatively advanced stage of growth. The cell line grew either as a monolayer on plastic dishes, as tumor spheroids in spinner culture, as lung nodules following injection of a single-cell suspension into the tail veins of syngeneic mice, or as a solid tumor. Both diploid and tetraploid clonogenic cells were found in monolayer cultures of the RIF-1 line.
开发了一种新的肿瘤模型系统（RIF-1），它非常适合用于以下研究：在这些研究中，比较各种治疗形式后的克隆形成存活率与生长延迟及控制概率。该肿瘤是近交系雌性C3H/Km小鼠中的辐射诱导肉瘤。它的中位肿瘤剂量较低，从体内直接到体外具有令人满意的接种效率，无免疫原性或免疫原性极小，并且仅在生长的相对晚期才发生转移。该细胞系既可以在塑料培养皿上形成单层生长，在旋转培养中形成肿瘤球体，将单细胞悬液注射到同基因小鼠尾静脉后形成肺结节，也可以形成实体瘤。在RIF-1细胞系的单层培养物中发现了二倍体和四倍体克隆形成细胞。
