Park C H, Amare M, Savin M A, Hoogstraten B
Cancer Res. 1980 Apr;40(4):1062-5.
The suppressive effect of L-ascorbic acid on the growth of bone marrow cells from patients with acute nonlymphocytic leukemia was studied using a modified agar culture method featuring daily feeding to allow the growth of leukemic cell colonies. In seven of 28 patients (25%), the numbers of leukemic cell colonies grown in culture were reduced to 21% of control by the addition of L-ascorbic acid (0.3 mM) to the culture medium. Glutathione did not suppress leukemic cell colonies although it has a similar oxidation-reduction potential to that of L-ascorbic acid. The addition of L-ascorbic acid reduced the pH of the medium. However, a comparable reduction of pH by the addition of HCl did not suppress leukemic cell colonies. In simultaneous cultures for leukemic and normal marrow cells, the suppression of leukemic cell colony was noted with a concentration of L-ascorbic acid as low as 0.1 mM (a concentration achievable in vivo), but normal myeloid colonies were not suppressed until the concentration of L-ascorbic acid reached an extremely high level (1 mM). In conclusion, growth of leukemic cells in culture was suppressed by L-ascorbic acid in a substantial proportion of patients with acute nonlymphocytic leukemia. This suppression was a specific effect of L-ascorbic acid and was not due to its oxidation-reduction potential or pH change. Leukemic cells were selectively affected at an L-ascorbic acid concentration attainable in vivo while normal hemopoietic cells were not suppressed.
采用改良琼脂培养法，通过每日换液以促进白血病细胞集落生长，研究了L-抗坏血酸对急性非淋巴细胞白血病患者骨髓细胞生长的抑制作用。在28例患者中的7例（25%），向培养基中添加L-抗坏血酸（0.3 mM）后，培养中生长的白血病细胞集落数量降至对照组的21%。谷胱甘肽虽然其氧化还原电位与L-抗坏血酸相似，但并未抑制白血病细胞集落。添加L-抗坏血酸会降低培养基的pH值。然而，通过添加HCl使pH值产生类似程度的降低并未抑制白血病细胞集落。在白血病细胞与正常骨髓细胞的同步培养中，发现L-抗坏血酸浓度低至0.1 mM（体内可达到的浓度）时即可抑制白血病细胞集落，但直到L-抗坏血酸浓度达到极高水平（1 mM）时才会抑制正常髓系集落。总之，在相当一部分急性非淋巴细胞白血病患者中，L-抗坏血酸可抑制培养中的白血病细胞生长。这种抑制是L-抗坏血酸的特异性作用，并非因其氧化还原电位或pH值变化所致。在体内可达到的L-抗坏血酸浓度下，白血病细胞受到选择性影响，而正常造血细胞未被抑制。
