Davis A R, Hiti A L, Nayak D P
Proc Natl Acad Sci U S A. 1980 Jan;77(1):215-9. doi: 10.1073/pnas.77.1.215.
The 3'- and 5'-terminal nucleotide sequences of the defective interfering (DI) RNAs present in a preparation of DI influenza virus were determined. It was found that all DI RNAs possessed identical terminal sequences for at least the first 13 nucleotides at the 5' end and at least the last 12 nucleotides at the 3' end. The sequence of the DI RNAs is (5')A-G-U-A-G-A-A-A-C-A-A-G-G-...-C-C-U-G-C-U-U-U-C-G-C-U-OH(3'). In addition, the same sequences were present at the 3' and 5' termini of the viral polymerase genes (P1, P2, and P3) from which these DI RNAs originate. These results indicate that DI RNAs of influenzing virus are formed by an internal deletion of the genomic RNA.
测定了存在于缺陷干扰（DI）流感病毒制剂中的DI RNA的3'和5'末端核苷酸序列。发现所有DI RNA在5'端至少前13个核苷酸以及3'端至少最后12个核苷酸处具有相同的末端序列。DI RNA的序列为（5'）A-G-U-A-G-A-A-A-C-A-A-G-G-...-C-C-U-G-C-U-U-U-C-G-C-U-OH（3'）。此外，这些DI RNA所源自的病毒聚合酶基因（P1、P2和P3）的3'和5'末端也存在相同的序列。这些结果表明，流感病毒的DI RNA是由基因组RNA的内部缺失形成的。
