Sega E, Mottolese M, Curcio C G, Citro G
J Natl Cancer Inst. 1980 May;64(5):1001-6.
A human lung tumor fetal-associated antigen (LTFA) has been purified from lung tumor tissue by a combination of salt precipitation and ion-exchange chromatography. The purification steps were monitored by double immunodiffusion with the use of a rabbit anti-LTFA-specific antiserum. The isolated protein was tested for its blastogenic properties toward peripheral blood lymphocytes (PBL) by [3H]thymidine incorporation. PBL obtained from 25 healthy individuals, 34 patients with tumors other than lung tumors, 13 patients with lung diseases other than lung tumors, and 51 lung tumor-bearing patients were tested. Only PBL with an in vitro positive response to phytohemagglutinin were employed. Whereas PBL of lung tumor patients showed a significant blastogenic response to the purified antigen in 14 of 22 patients tested (60%), PBL of other patients were completely unreactive (P less than 0.005). The present data suggested that a specific immune response toward an LTFA was present in patients with lung cancers.
一种人肺肿瘤胎儿相关抗原（LTFA）已通过盐沉淀和离子交换色谱相结合的方法从肺肿瘤组织中纯化出来。纯化步骤通过使用兔抗LTFA特异性抗血清的双向免疫扩散进行监测。通过[3H]胸苷掺入法检测分离出的蛋白质对外周血淋巴细胞（PBL）的促有丝分裂特性。对从25名健康个体、34名非肺肿瘤患者、13名非肺肿瘤性肺部疾病患者和51名肺癌患者中获得的PBL进行了检测。仅使用对植物血凝素体外呈阳性反应的PBL。在测试的22名患者中，有14名（60%）肺癌患者的PBL对纯化抗原表现出显著的促有丝分裂反应，而其他患者的PBL则完全无反应（P<0.005）。目前的数据表明，肺癌患者中存在针对LTFA的特异性免疫反应。
