Blatteis C M, Cardoso S S, Narayanan T K, Hughes M H, Morris H P
J Natl Cancer Inst. 1980 Jun;64(6):1451-8. doi: 10.1093/jnci/64.6.1451.
Female inbred BUF rats bearing Morris hepatomas 5123C, 5123D, 7795, and 7800 bilaterally in the femoral musculature were exposed for 3 weeks to ether 4,500-m simulated altitude or sea level or to an ambient temperature of either 7, 23, or 33 degrees C. Rats were given inoculations 12 days before these exposures. Tumor size, body weight, food consumption, and body temperatures were measured weekly in these treated rats and in normal rats. At time of killing, tumor mass, DNA synthesis (by [3H]thymidine incorporation), and respiration (by conversion of [1,4-14C]succinic acid to 14CO2) were measured in each of the 4 hepatoma lines, in the livers of normal and host rats, and in regenerated livers 10 days post 70% hepatectomy. Growths of all 4 tumors and regenerated livers were significantly impaired in rats stressed by exposure to altitude and heat but not to cold. Neither DNA synthesis nor respiration was altered in the hepatomas and livers by any environmental stress. The environmentally stressed rats gained weight at a slower rate and consumed less food than did their controls, but no differences were found in these variables for tumor-bearing and non-tumor-bearing rats. However, whereas the ratio of body weight gain to food consumed was reduced under the three stressful environments, that of tumor weight gain to food consumed was not altered by any environment. Host survivorship was not influenced by any of these effects.
在双侧股部肌肉中接种了莫氏肝癌5123C、5123D、7795和7800的雌性近交BUF大鼠，分别暴露于海拔4500米模拟高度的乙醚环境、海平面环境或7℃、23℃或33℃的环境温度下3周。在这些暴露前12天给大鼠接种。每周测量这些处理大鼠和正常大鼠的肿瘤大小、体重、食物摄入量和体温。处死时，在4种肝癌细胞系、正常大鼠和宿主大鼠的肝脏以及70%肝切除术后10天的再生肝脏中，测量肿瘤质量、DNA合成（通过[3H]胸腺嘧啶核苷掺入）和呼吸作用（通过[1,4-14C]琥珀酸转化为14CO2）。暴露于海拔和高温而非低温应激的大鼠中，所有4种肿瘤和再生肝脏的生长均受到显著损害。任何环境应激均未改变肝癌和肝脏中的DNA合成及呼吸作用。与对照组相比，环境应激大鼠体重增加速度较慢，食物摄入量较少，但在这些变量方面，荷瘤大鼠和非荷瘤大鼠之间未发现差异。然而，在三种应激环境下，体重增加与食物摄入量的比值降低，而肿瘤重量增加与食物摄入量的比值未因任何环境而改变。宿主的存活率不受这些影响中的任何一种影响。
