人类细胞中H4组蛋白mRNA的多种形式。
Multiple forms of H4 histone mRNA in human cells.
作者信息
Lichtler A C, Detke S, Phillips I R, Stein G S, Stein J L
出版信息
Proc Natl Acad Sci U S A. 1980 Apr;77(4):1942-6. doi: 10.1073/pnas.77.4.1942.
Abstract
Two species of H4 histone mRNA were isolated from the polysomes of S phase HeLa S3 cells. Electrophoresis under denaturing and nondenaturing conditions indicates that the two H4 mRNAs differ in size. Both mRNAs translate H4 histones in vitro, lack poly(A) at their 3' termini, and are capped at their 5' termini. Polyacrylamide gel electrophoresis and tryptic peptide analysis suggest that the polypeptides synthesized by the two mRNAs are indistinguishable.
摘要
从S期海拉S3细胞的多核糖体中分离出两种H4组蛋白mRNA。变性和非变性条件下的电泳表明这两种H4 mRNA大小不同。两种mRNA在体外均能翻译H4组蛋白，其3'末端缺乏聚腺苷酸（poly(A)），5'末端有帽结构。聚丙烯酰胺凝胶电泳和胰蛋白酶肽分析表明，由这两种mRNA合成的多肽无法区分。
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