采用带有电子捕获检测的气液色谱法测定大鼠脑区中的5-羟色胺。

Determination of 5-hydroxytryptamine in rat brain regions by gas-liquid chromatography with electron-capture detection.

作者信息

Baker G B, Martin I L, Coutts R T, Benderly A

出版信息

J Pharmacol Methods. 1980 Feb;3(2):173-9. doi: 10.1016/0160-5402(80)90027-3.

Abstract

An assay method for measurement of 5-hydroxytryptamine in rat brain areas is described. The procedure involves extraction with a liquid ion exchanger followed by acetylation and reaction with pentafluoropropionic anhydride. The resultant beta-carboline is separated and quantitated using gas-liquid chromatography with electron-capture detection. The procedure also works well with 5-methoxytryptamine standards, and the tissue samples were checked simultaneously for the presence of this amine. The results indicate that concentrations of 5-methoxytryptamine in all areas studied (brain stem, hypothalamus, frontal cortex, and striatum) are less than 10 ng/g.

摘要

本文描述了一种测定大鼠脑区中5-羟色胺的分析方法。该方法包括用液体离子交换剂萃取，然后进行乙酰化，并与五氟丙酸酐反应。所得的β-咔啉通过带有电子捕获检测的气液色谱法进行分离和定量。该方法对5-甲氧基色胺标准品也同样适用，并且同时对组织样本中该胺的存在情况进行了检测。结果表明，在所研究的所有脑区（脑干、下丘脑、额叶皮质和纹状体）中，5-甲氧基色胺的浓度均低于10 ng/g。