• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

噬菌体T7 DNA复制一级和二级起始位点的物理图谱

Physical mapping of primary and secondary origins of bacteriophage T7 DNA replication.

作者信息

Tamanoi F, Saito H, Richardson C C

出版信息

Proc Natl Acad Sci U S A. 1980 May;77(5):2656-60. doi: 10.1073/pnas.77.5.2656.

DOI:10.1073/pnas.77.5.2656
PMID:6930658
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC349461/
Abstract

Deletion mutants of bacteriophage T7 have been used to identify and to map, by electron microscopy, the origins of T7 DNA replication. The primary origin of phage T7 DNA replication lies within a 100-base-pair region located 14.75-15.0% of the distance from the genetic left end of the DNA molecule. T7 phage whose DNA contains a deletion of this region initiate replication at secondary origins, the predominant one of which is located at a distance approximately 4% from the left end of the molecule.

摘要

噬菌体T7的缺失突变体已被用于通过电子显微镜鉴定和绘制T7 DNA复制的起始位点。噬菌体T7 DNA复制的主要起始位点位于一个100个碱基对的区域内，该区域位于DNA分子遗传左端距离的14.75 - 15.0%处。其DNA包含该区域缺失的T7噬菌体在次要起始位点启动复制，其中主要的次要起始位点位于距离分子左端约4%处。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6508/349461/1bdd607f441a/pnas00492-0314-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6508/349461/2725c05454db/pnas00492-0312-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6508/349461/1bdd607f441a/pnas00492-0314-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6508/349461/2725c05454db/pnas00492-0312-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6508/349461/1bdd607f441a/pnas00492-0314-a.jpg

相似文献

1
Physical mapping of primary and secondary origins of bacteriophage T7 DNA replication.噬菌体T7 DNA复制一级和二级起始位点的物理图谱
Proc Natl Acad Sci U S A. 1980 May;77(5):2656-60. doi: 10.1073/pnas.77.5.2656.
2
Nucleotide sequence of the primary origin of bacteriophage T7 DNA replication: relationship to adjacent genes and regulatory elements.噬菌体T7 DNA复制起始位点的核苷酸序列：与相邻基因及调控元件的关系
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3917-21. doi: 10.1073/pnas.77.7.3917.
3
In vivo analysis of the initiation of bacteriophage T7 DNA replication.噬菌体T7 DNA复制起始的体内分析
Virology. 1990 Feb;174(2):585-92. doi: 10.1016/0042-6822(90)90111-4.
4
Initiation of DNA replication at cloned origins of bacteriophage T7.噬菌体T7克隆起始点处DNA复制的起始
J Mol Biol. 1988 Dec 20;204(4):903-16. doi: 10.1016/0022-2836(88)90050-2.
5
Bacteriophage T7 DNA packaging. I. Plasmids containing a T7 replication origin and the T7 concatemer junction are packaged into transducing particles during phage infection.噬菌体T7 DNA包装。I. 在噬菌体感染期间，含有T7复制起点和T7多联体连接点的质粒被包装到转导颗粒中。
J Mol Biol. 1990 Dec 20;216(4):911-26. doi: 10.1016/S0022-2836(99)80010-2.
6
Initiation of DNA replication at the primary origin of bacteriophage T7 by purified proteins: requirement for T7 RNA polymerase.利用纯化蛋白在噬菌体T7的主要起始位点启动DNA复制：对T7 RNA聚合酶的需求
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4107-11. doi: 10.1073/pnas.78.7.4107.
7
Cloning of the T7 genome in Escherichia coli: use of recombination between cloned sequences and bacteriophage T7 to identify genes involved in recombination and a clone containing the origin of T7 DNA replication.T7基因组在大肠杆菌中的克隆：利用克隆序列与噬菌体T7之间的重组来鉴定参与重组的基因以及一个含有T7 DNA复制起点的克隆。
Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:441-8. doi: 10.1101/sqb.1979.043.01.050.
8
Initiation of DNA replication at the primary origin of bacteriophage T7 by purified proteins. Site and direction of initial DNA synthesis.利用纯化蛋白在噬菌体T7的主要起始点引发DNA复制。初始DNA合成的位点和方向。
J Biol Chem. 1985 Mar 10;260(5):3185-96.
9
Bacteriophage T7 DNA packaging. II. Analysis of the DNA sequences required for packaging using a plasmid transduction assay.噬菌体T7 DNA包装。II. 使用质粒转导测定法分析包装所需的DNA序列。
J Mol Biol. 1990 Dec 20;216(4):927-38. doi: 10.1016/S0022-2836(99)80011-4.
10
Change in priming sites for discontinuous DNA synthesis between the monomeric and concatemeric stages of phage T7 replication.噬菌体T7复制的单体阶段和多联体阶段之间不连续DNA合成引发位点的变化。
Mol Gen Genet. 1988 Mar;211(3):400-6. doi: 10.1007/BF00425692.

引用本文的文献

1
Gp2.5, the multifunctional bacteriophage T7 single-stranded DNA binding protein.Gp2.5，多功能噬菌体 T7 单链 DNA 结合蛋白。
Semin Cell Dev Biol. 2019 Feb;86:92-101. doi: 10.1016/j.semcdb.2018.03.018. Epub 2018 Mar 28.
2
Refactoring bacteriophage T7.改造噬菌体T7
Mol Syst Biol. 2005;1:2005.0018. doi: 10.1038/msb4100025. Epub 2005 Sep 13.
3
Evolutionary role of abortive transcript as a primer for DNA replication.流产转录本作为DNA复制引物的进化作用。

本文引用的文献

1
Recognition and initiation site for four late promoters of phage T7 is a 22-base pair DNA sequence.噬菌体T7四个晚期启动子的识别和起始位点是一段22个碱基对的DNA序列。
Nature. 1979 Jul 5;280(5717):35-9. doi: 10.1038/280035a0.
2
The genetics and physiology of bacteriophage T7.噬菌体T7的遗传学与生理学
Virology. 1969 Nov;39(3):562-74. doi: 10.1016/0042-6822(69)90104-4.
3
Physical mapping of the early region of bacteriophage T7 DNA.噬菌体T7 DNA早期区域的物理图谱
J Mol Evol. 1994 Dec;39(6):620-4. doi: 10.1007/BF00160407.
4
Genetic analysis of gene 1.2 of bacteriophage T7: isolation of a mutant of Escherichia coli unable to support the growth of T7 gene 1.2 mutants.噬菌体T7基因1.2的遗传分析：分离出一株无法支持T7基因1.2突变体生长的大肠杆菌突变体。
J Virol. 1981 Jan;37(1):343-51. doi: 10.1128/JVI.37.1.343-351.1981.
5
Initiation of DNA replication at the primary origin of bacteriophage T7 by purified proteins: requirement for T7 RNA polymerase.利用纯化蛋白在噬菌体T7的主要起始位点启动DNA复制：对T7 RNA聚合酶的需求
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4107-11. doi: 10.1073/pnas.78.7.4107.
6
Genetic analysis of two bacterial RNA polymerase mutants that inhibit the growth of bacteriophage T7.对两种抑制噬菌体T7生长的细菌RNA聚合酶突变体的遗传分析。
Mol Gen Genet. 1982;188(2):211-8. doi: 10.1007/BF00332677.
7
Template recognition sequence for RNA primer synthesis by gene 4 protein of bacteriophage T7.噬菌体T7基因4蛋白合成RNA引物的模板识别序列。
Proc Natl Acad Sci U S A. 1981 Jan;78(1):205-9. doi: 10.1073/pnas.78.1.205.
8
Multiply branched replicative intermediates in E. coli and bacteriophage lambda.大肠杆菌和噬菌体λ中的多分支复制中间体
Mol Gen Genet. 1981;184(3):450-6. doi: 10.1007/BF00352521.
9
Viable deletions of the M13 complementary strand origin.M13互补链起始位点的可行缺失。
Proc Natl Acad Sci U S A. 1981 Nov;78(11):6784-8. doi: 10.1073/pnas.78.11.6784.
10
Initiation sites for discontinuous DNA synthesis of bacteriophage T7.噬菌体T7不连续DNA合成的起始位点。
Proc Natl Acad Sci U S A. 1981 Feb;78(2):903-7. doi: 10.1073/pnas.78.2.903.
J Mol Biol. 1973 Sep 15;79(2):249-65. doi: 10.1016/0022-2836(73)90004-1.
4
Multiple and specific initiation of T4 DNA replication.T4 DNA复制的多重特异性起始
J Virol. 1973 Jul;12(1):130-48. doi: 10.1128/JVI.12.1.130-148.1973.
5
Initiation and reinitiation of DNA synthesis during replication of bacteriophage T7.噬菌体T7复制过程中DNA合成的起始与重新起始
Proc Natl Acad Sci U S A. 1972 Apr;69(4):998-1002. doi: 10.1073/pnas.69.4.998.
6
Bacteriophage T7 DNA replication: a linear replicating intermediate (gradient centrifugation-electron microscopy-E. coli-DNA partial denaturation).噬菌体T7 DNA复制：一种线性复制中间体（梯度离心-电子显微镜-大肠杆菌-DNA部分变性）
Proc Natl Acad Sci U S A. 1972 Feb;69(2):499-504. doi: 10.1073/pnas.69.2.499.
7
Presence of displacement loops in the covalently closed circular chloroplast deoxyribonucleic acid from higher plants.高等植物共价闭合环状叶绿体脱氧核糖核酸中置换环的存在。
J Biol Chem. 1975 Nov 25;250(22):8840-7.
8
Bacteriophage T7 genetics.噬菌体T7遗传学
Curr Top Microbiol Immunol. 1976;75:77-110. doi: 10.1007/978-3-642-66530-1_3.
9
Gene 2 protein of bacteriophage T7: purification and requirement for packaging of T7 DNA in vitro.噬菌体T7的基因2蛋白：体外纯化及T7 DNA包装的需求
Proc Natl Acad Sci U S A. 1979 Oct;76(10):4852-6. doi: 10.1073/pnas.76.10.4852.
10
Initiation of DNA synthesis in Escherichia coli.大肠杆菌中DNA合成的起始
Annu Rev Biochem. 1979;48:999-1034. doi: 10.1146/annurev.bi.48.070179.005031.