Corasanti J G, Celik C, Camiolo S M, Mittelman A, Evers J L, Barbasch A, Hobika G H, Markus G
J Natl Cancer Inst. 1980 Aug;65(2):345-51.
Plasminogen activator content was determined quantitatively in extracts of 23 pairs of surgically removed colon tumors and adjacent normal mucosa specimens. The activator content averaged 4.4 times higher in the tumor samples than in the corresponding normal tissue. Polyps removed with the adenocarcinomas gave values intermediate between those for tumors and those for the normal mucosae. The enzyme content of the group of tumors that showed invasive propagation or metastatic spread was significantly higher (P < 0.05) than was the enzyme content of the group not manifesting these conditions. Activator activity of the tumor extracts was completely inhibited by rabbit antibody formed against human urokinse. The activity of the normal mucosae was variably inhibited, suggesting the presence of several kinds of activator in normal tissues. The activator from the normal tissues could be separated into a completely refractory fraction and a completely inhibitable fraction by means of an affinity column made of Sepharose-linked, rabbit antiurokinase antibody. The activator, eluted from this column with 1 M acetic acid in 0.5 M NaCl (pH 2.2), was highly purified and had an isoelectric point of 8.6, as does authentic urokinase. The details of the isoelectric profile of the two, however, differed. The data are discussed in relation to earlier studies on the fibrinolytic system in colon cancer.
对23对手术切除的结肠肿瘤及相邻正常黏膜标本的提取物进行了纤溶酶原激活物含量的定量测定。肿瘤样本中的激活物含量平均比相应正常组织高4.4倍。与腺癌一并切除的息肉的值介于肿瘤和正常黏膜的值之间。表现为浸润性生长或转移扩散的肿瘤组的酶含量显著高于（P < 0.05）未表现出这些情况的肿瘤组的酶含量。肿瘤提取物的激活物活性被抗人尿激酶的兔抗体完全抑制。正常黏膜的活性受到不同程度的抑制，这表明正常组织中存在几种激活物。通过由交联琼脂糖的兔抗尿激酶抗体制成的亲和柱，可将正常组织中的激活物分离为完全耐受部分和完全可抑制部分。用0.5 M NaCl（pH 2.2）中的1 M乙酸从该柱上洗脱下来的激活物高度纯化，其等电点为8.6，与纯尿激酶相同。然而，两者的等电图谱细节有所不同。结合早期关于结肠癌纤维蛋白溶解系统的研究对这些数据进行了讨论。
