van Driel-Kulker A M, Meyer F, Ploem J S
Microsc Acta Suppl. 1980;Suppl 4:73-81.
The Leyden Television Analysis System (LEYTAS) is applied to the automated analysis of five positive specimens (2 cases of severe dysplasia, 2 cases of carcinoma in situ and 1 case of invasive carcinoma) and five negative specimens (4 cases of inflammation and 1 normal specimen). Two detection criteria based on size and contrast (so called 'top hat' transformation) are used to detect suspect nuclei. Thresholds and sizes have been used at two levels: 'high level' to detect very large and/or dark nuclei, and 'low level' to detect nuclei, of which absorption and/or size is only slightly increased compared to the nuclei of normal epithelial cells. In all positive specimens analysed with the 'high level' program suspect nuclei are automatically detected, whereas no nuclei are detected in any of the negative specimens. Using the 'low level' program many more suspect nuclei are detected in the positive specimens (up to a number of 294). This program results in some detected nuclei in the negative specimens, but the number remains relatively low (not more than 3 in the investigated cases).
莱顿电视分析系统（LEYTAS）被应用于对5份阳性标本（2例重度发育异常、2例原位癌和1例浸润癌）和5份阴性标本（4例炎症和1例正常标本）进行自动分析。基于大小和对比度的两种检测标准（即所谓的“顶帽”变换）被用于检测可疑细胞核。阈值和大小被设置为两个水平：“高水平”用于检测非常大的和/或深色的细胞核，“低水平”用于检测与正常上皮细胞核相比，吸收和/或大小仅略有增加的细胞核。在用“高水平”程序分析的所有阳性标本中，可疑细胞核均被自动检测到，而在任何阴性标本中均未检测到细胞核。使用“低水平”程序时，在阳性标本中检测到更多可疑细胞核（多达294个）。该程序在阴性标本中也检测到了一些细胞核，但数量仍然相对较少（在所研究的病例中不超过3个）。
