Fontana J A, Wright D G, Schiffman E, Corcoran B A, Deisseroth A B
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3664-8. doi: 10.1073/pnas.77.6.3664.
We have studied the events that occur during the development of chemotaxis in HL60, a promyelocytic leukemia cell line that acquires the features of mature neutrophils when exposed to dimethylformamide (DMF). Chemotactic function first appears between 48 and 96 hr of DMF induction and is associated not only with the coincidental development of deformability, spontaneous motility, greatly increased binding of fMet-Leu-Phe, and orientation but also with decreasing cell size and pleomorphism of nuclei. Surface adhesiveness develops earlier (36-48 hr) and is coincident with a 10-fold increase in protein synthesis not seen in other DMF-inducible cell lines. This burst of protein synthesis precedes the expression of chemotactic function. These studies show that the HL60 cell line can provide a useful model for delineating control mechanisms responsible for the development of complex cellular functions present in differentiated myeloid cells in humans.
我们研究了HL60细胞系趋化性发育过程中发生的事件。HL60是一种早幼粒细胞白血病细胞系，当暴露于二甲基甲酰胺（DMF）时，它会获得成熟中性粒细胞的特征。趋化功能首先在DMF诱导的48至96小时之间出现，不仅与同时出现的可变形性、自发运动性、fMet-Leu-Phe结合显著增加以及定向有关，还与细胞大小减小和细胞核多形性有关。表面黏附性出现得更早（36 - 48小时），并且与蛋白质合成增加10倍同时发生，这在其他DMF诱导的细胞系中未见。这种蛋白质合成的爆发先于趋化功能的表达。这些研究表明，HL60细胞系可以为描绘负责人类分化髓细胞中复杂细胞功能发育的控制机制提供一个有用的模型。
