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33258 Hoechst enhancement of the photosensitivity of bromodeoxyuridine-substituted cells.

作者信息

Stetten G, Latt S A, Davidson R L

出版信息

Somatic Cell Genet. 1976 May;2(3):285-90. doi: 10.1007/BF01538967.

DOI:10.1007/BF01538967
PMID:69327
Abstract

Cells that have incorporated 5-bromodeoxyuridine (BrdU) into their DNA become extremely sensitive to photoinduced killing after treatment with the dye, 33258 Hoechst. Under appropriate conditions the dye increases the efficiency of killing by a factor of approximately 10(4). This enhanced photosensitivity suggests that the combination of BrdU, dye, and light can be used to select conditional lethal mutants.

摘要

相似文献

1
33258 Hoechst enhancement of the photosensitivity of bromodeoxyuridine-substituted cells.
Somatic Cell Genet. 1976 May;2(3):285-90. doi: 10.1007/BF01538967.
2
33258 Hoechst enhances the selectivity of the bromodeoxyuridine--light method of isolating conditional lethal mutants.
Exp Cell Res. 1977 Sep;108(2):447-52. doi: 10.1016/s0014-4827(77)80055-4.
3
Photochemistry of the bisbenzimidazole dye 33258 Hoechst with bromodeoxyuridine and its biological effects on Brd Urd-substituted Escherichia coli.双苯并咪唑染料33258 Hoechst与溴脱氧尿苷的光化学及其对溴脱氧尿苷取代的大肠杆菌的生物学效应。
Photochem Photobiol. 1978 May;27(5):559-63. doi: 10.1111/j.1751-1097.1978.tb07646.x.
4
The interaction of Hoechst 33258 and BrdU substituted DNA in the formation of sister chromatid exchanges.Hoechst 33258与BrdU取代的DNA在姐妹染色单体交换形成中的相互作用。
Chromosoma. 1977 Aug 18;63(1):21-31. doi: 10.1007/BF00292939.
5
The action spectrum (313-435 nm) for killing Hoechst 33258 treated Chinese hamster ovary cells containing bromodeoxyuridine substituted DNA.
Photochem Photobiol. 1982 Feb;35(2):163-6. doi: 10.1111/j.1751-1097.1982.tb03826.x.
6
Isolation of thymidine kinase-deficient rat hepatoma cells by selection with bromodeoxyuridine, Hoechst 33258, and visible light.通过用溴脱氧尿苷、Hoechst 33258和可见光进行筛选来分离胸苷激酶缺陷型大鼠肝癌细胞。
Biochem Genet. 1984 Apr;22(3-4):201-13. doi: 10.1007/BF00484224.
7
Response of bromodeoxyuridine-substituted Chinese hamster cells to UVA light exposure in the presence of Hoechst dye #33258: survival and DNA repair studies.在存在 Hoechst 染料 #33258 的情况下，溴脱氧尿苷取代的中国仓鼠细胞对紫外线 A 照射的反应：存活与 DNA 修复研究。
Radiat Res. 1994 Jun;138(3):312-9.
8
Hoechst 33258 dye generates DNA-protein cross-links during ultraviolet light-induced photolysis of bromodeoxyuridine in replicated and repaired DNA.在复制和修复的DNA中，溴脱氧尿苷经紫外线诱导光解时，Hoechst 33258染料会产生DNA-蛋白质交联。
Photochem Photobiol. 1986 Aug;44(2):131-6. doi: 10.1111/j.1751-1097.1986.tb03576.x.
9
Application of the BrdU/thymidine method to flow cytogenetics: differential quenching/enhancement of Hoechst 33258 fluorescence of late-replicating chromosomes.5-溴脱氧尿苷/胸腺嘧啶核苷法在流式细胞遗传学中的应用：晚期复制染色体上Hoechst 33258荧光的差异猝灭/增强
Somatic Cell Genet. 1982 May;8(3):319-27. doi: 10.1007/BF01538890.
10
Flow cytometric analysis of bromodeoxyuridine-substituted cells stained with 33258 Hoechst.用33258 Hoechst对溴脱氧尿苷取代细胞进行流式细胞术分析。
J Histochem Cytochem. 1977 Jul;25(7):927-34. doi: 10.1177/25.7.70460.

引用本文的文献

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Nucleic Acids Res. 1993 Jul 11;21(14):3227-32. doi: 10.1093/nar/21.14.3227.
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Revertants of an S49 cell mutant that expresses altered cyclic AMP-dependent protein kinase.表达改变的环磷酸腺苷依赖性蛋白激酶的S49细胞突变体的回复突变体。
Mol Cell Biol. 1982 Oct;2(10):1229-37. doi: 10.1128/mcb.2.10.1229-1237.1982.
5
Isolation of thymidine kinase-deficient rat hepatoma cells by selection with bromodeoxyuridine, Hoechst 33258, and visible light.通过用溴脱氧尿苷、Hoechst 33258和可见光进行筛选来分离胸苷激酶缺陷型大鼠肝癌细胞。
Biochem Genet. 1984 Apr;22(3-4):201-13. doi: 10.1007/BF00484224.
6
Immunoreactive myelin basic proteins are not detected when shiverer mutant Schwann cells and fibroblasts are co-cultured with normal neurons.当颤抖突变型雪旺细胞和成纤维细胞与正常神经元共培养时，未检测到免疫反应性髓鞘碱性蛋白。
J Cell Biol. 1984 Apr;98(4):1291-5. doi: 10.1083/jcb.98.4.1291.
7
UV dose-dependent increase in the Hoechst fluorescence intensity of both normal and BrdU-DNA.正常和BrdU-DNA的Hoechst荧光强度均随紫外线剂量增加。
Histochemistry. 1982;74(2):279-91. doi: 10.1007/BF00495837.
8
Identification of temperature-sensitive DNA- mutants of Chinese hamster cells affected in cellular and viral DNA synthesis.中国仓鼠细胞中影响细胞和病毒DNA合成的温度敏感型DNA突变体的鉴定。
Mol Cell Biol. 1986 Dec;6(12):4594-601. doi: 10.1128/mcb.6.12.4594-4601.1986.
9
The interaction of Hoechst 33258 and BrdU substituted DNA in the formation of sister chromatid exchanges.Hoechst 33258与BrdU取代的DNA在姐妹染色单体交换形成中的相互作用。
Chromosoma. 1977 Aug 18;63(1):21-31. doi: 10.1007/BF00292939.