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哺乳动物细胞中生长抑制序列的基因选择。

Genetic selection of growth-inhibitory sequences in mammalian cells.

作者信息

Pestov D G, Lau L F

机构信息

Department of Genetics, University of Illinois College of Medicine, Chicago 60612.

出版信息

Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12549-53. doi: 10.1073/pnas.91.26.12549.

DOI:10.1073/pnas.91.26.12549
PMID:7809075
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC45476/
Abstract

To assess the role of mitogenically activated genes in the control of cell proliferation, we have taken a genetic approach based on the premise that blocking the function of an essential gene should lead to growth inhibition. Using a newly developed selection procedure, we isolated growth-inhibitory sequences from a pool of random cDNA fragments of 19 growth-related genes associated with the G0/G1 transition. These sequences encode potential dominant negative variants of c-Fos, JunB, and p44MAPK that may interfere with their growth-related functions. We anticipate that this procedure, which allows for the selection of sequences that cause a growth-inhibition phenotype, may have broad applications in the identification and analysis of genes that regulate cell growth.

摘要

为了评估有丝分裂原激活基因在控制细胞增殖中的作用，我们采用了一种基于以下前提的遗传学方法：阻断一个必需基因的功能应导致生长抑制。利用新开发的筛选程序，我们从与G0/G1期转换相关的19个生长相关基因的随机cDNA片段库中分离出了生长抑制序列。这些序列编码可能干扰其生长相关功能的c-Fos、JunB和p44MAPK的潜在显性负变体。我们预计，这种能够筛选出导致生长抑制表型序列的程序，可能在调节细胞生长的基因的鉴定和分析中具有广泛的应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c7/45476/66f080ff0db4/pnas01477-0207-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c7/45476/cea2d75ad02e/pnas01477-0205-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c7/45476/1c546eaa5ecc/pnas01477-0206-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c7/45476/327faf64a67f/pnas01477-0207-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c7/45476/66f080ff0db4/pnas01477-0207-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c7/45476/cea2d75ad02e/pnas01477-0205-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c7/45476/1c546eaa5ecc/pnas01477-0206-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c7/45476/327faf64a67f/pnas01477-0207-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c7/45476/66f080ff0db4/pnas01477-0207-b.jpg

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1
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Oncogene. 1993 Mar;8(3):797-801.
2
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Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8319-23. doi: 10.1073/pnas.90.18.8319.
3
The MAP kinase cascade is essential for diverse signal transduction pathways.
牛病毒性腹泻病毒进入的反式显性抑制
J Virol. 2008 Mar;82(5):2427-36. doi: 10.1128/JVI.02158-07. Epub 2007 Dec 19.
4
Covert genetic selections to optimize phenotypes.进行隐蔽的基因筛选以优化表型。
PLoS One. 2007 Nov 21;2(11):e1200. doi: 10.1371/journal.pone.0001200.
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Restricting conformational flexibility of the switch II region creates a dominant-inhibitory phenotype in Obg GTPase Nog1.限制开关II区域的构象灵活性会在Obg GTP酶Nog1中产生显性抑制表型。
Mol Cell Biol. 2007 Nov;27(21):7735-44. doi: 10.1128/MCB.01161-07. Epub 2007 Sep 4.
6
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Nucleic Acids Res. 2006 May 31;34(10):3030-43. doi: 10.1093/nar/gkl378. Print 2006.
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8
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9
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4
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5
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6
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7
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8
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9
Translocation, breakage and truncated transcripts of c-myc oncogene in murine plasmacytomas.小鼠浆细胞瘤中c-myc癌基因的易位、断裂及截短转录本
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10
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