Hermens A F
Br J Cancer Suppl. 1980 Apr;4:245-50.
In order to study possible variations in the expression of the clonogenic capacity of cultured R-1,M tumour cells due to different conditions of the growth substrate, assays were performed by employing the in vitro plating, technique described by Puck & Marcus (1956) and the in vivo TD50 assay developed by Hewitt & Wilson (1959). Assays were performed with cell suspensions containing R-1,M cells alone or admixed with either heavily irradiated R-1,M cells designated as F(R-1,M) cells or normal, syngeneic MER-1 cells that have a phagocytic capacity. In vitro assays demonstrated a maximal capacity for colony formation of 80 to 100% of the R-1,M cells plated. TD50 assays performed with the syngeneic WAG/Rij rat and the allogeneic BALB/c.nu mouse revealed that R-1,M cells can express their clonogenic capacity in both strains equally well, with a TD50 of 6,000 cells. From results of assays performed with admixed cells, it was concluded that, in the BALB/c.nu mouse, MER-1 cells are capable of reducing the TD50 by a factor of 600, while admixture with both MER-1 and F(R-1,M) cells in the WAG/Rij rat resulted in a reduction by a factor of only 3-4. The intrinsic radiosensitivity of R-1,M cells grown in single cultures, and mixed cultures with MER-1 cells, was studied by the in vitro assay after in vitro irradiation. For R-1,M cells DQ and D0 values of 2.5 and 1.3 Gy, respectively, were obtained. However, in vivo assays for survival of in vitro irradiated R-1,M cells in single culture provided data which cannot be correlated in a simple manner with data obtained by the in vitro assay.
为了研究培养的R-1,M肿瘤细胞克隆形成能力的表达因生长底物条件不同而可能出现的变化，采用了Puck和Marcus（1956年）描述的体外接种技术以及Hewitt和Wilson（1959年）开发的体内TD50测定法进行检测。检测使用的细胞悬液中仅含有R-1,M细胞，或与经大量辐照的R-1,M细胞（称为F(R-1,M)细胞）或具有吞噬能力的同基因正常MER-1细胞混合。体外检测显示，接种的R-1,M细胞的最大集落形成能力为80%至100%。用同基因WAG/Rij大鼠和异基因BALB/c.nu小鼠进行的TD50测定表明，R-1,M细胞在这两种品系中均能同样良好地表达其克隆形成能力，TD50为每只动物6000个细胞。从混合细胞检测结果得出结论，在BALB/c.nu小鼠中，MER-1细胞能够使TD50降低600倍，而在WAG/Rij大鼠中，MER-1细胞与F(R-1,M)细胞混合后，TD50仅降低3至4倍。通过体外照射后的体外检测研究了单培养以及与MER-1细胞混合培养的R-1,M细胞的内在放射敏感性。对于R-1,M细胞，分别获得了2.5 Gy和1.3 Gy的DQ和D0值。然而，对体外照射的单培养R-1,M细胞进行的体内存活检测所提供的数据，无法与体外检测获得的数据进行简单关联。
