Suit H D, Rockwell S, Zeitman A, Silobrcic V, Porter E A, Ramsay J, Sedlacek R
Edwin L. Steele Laboratory for Radiation Biology, Department of Radiation Medicine, Massachusetts General Hospital, Harvard Medical School, Boston 02114.
Eur J Cancer Clin Oncol. 1989 Oct;25(10):1463-6. doi: 10.1016/0277-5379(89)90105-3.
Quantitative tumor cell transplantation assays have been performed to compare the transplantability of rat rhabdomyosarcoma BA1112 into isologous WAG/Rij Y rats and athymic NCr(nu/nu) nude mice. The end-point was the TD50 or the number of viable tumor cells which would transplant the tumors into half of the recipients. At Yale, two sets of 2-fold dilutions were prepared, one was sent to the MGH by Air Express. That afternoon, concurrent assays were performed at Yale using the WAG/Rij Y rat and at MGH using the NCr(nu/nu) mouse. The TD50 values were the same for iso- and xenotransplantation. Furthermore, the TD50s in rats and mice were unaffected by standard immunization procedures prior to challenge of the TD50 assay. The BA1112 (10(7) trypan blue excluding cells) grew to 10-12 mm and then completely regressed if transplanted into NCr(nu/+) mice which had received 6 Gy whole body irradiation but did not grow in control NCr(nu/+) mice. The times for the BA1112 to grow to 10 mm were the same in normal or preimmunized WAG/Rij Y rats or NCr(nu/nu) mice and in 6 Gy WBI NCr(nu/nu) mice. All of the experimental data show that the xenogenic NCr(nu/nu) mice accept the BA1112 as readily as do the isologous WAG/Rij Y rats.
已进行定量肿瘤细胞移植试验，以比较大鼠横纹肌肉瘤BA1112在同基因WAG/Rij Y大鼠和无胸腺NCr(nu/nu)裸鼠中的移植能力。终点指标是TD50，即能使一半受体发生肿瘤移植的活肿瘤细胞数量。在耶鲁大学，制备了两组2倍稀释液，一组通过航空快递送到了麻省总医院。当天下午，耶鲁大学使用WAG/Rij Y大鼠同时进行了试验，麻省总医院则使用NCr(nu/nu)小鼠进行了试验。同基因移植和异种移植的TD50值相同。此外，在进行TD50试验前，大鼠和小鼠的TD50不受标准免疫程序的影响。如果将BA1112（10(7)个台盼蓝拒染细胞）移植到接受6 Gy全身照射的NCr(nu/+)小鼠中，其可生长至10 - 12 mm，然后完全消退，但在对照NCr(nu/+)小鼠中则不生长。在正常或预先免疫的WAG/Rij Y大鼠或NCr(nu/nu)小鼠以及接受6 Gy全身照射的NCr(nu/nu)小鼠中，BA1112生长至10 mm的时间相同。所有实验数据表明，异种NCr(nu/nu)小鼠接受BA1112的程度与同基因WAG/Rij Y大鼠一样容易。
