Curtis S B, Tenforde T S
Br J Cancer Suppl. 1980 Apr;4:266-70.
A rhabdomyosarcoma in a WAG/Rij rat with capacity for colony growth after tumour excision and enzymatic dissociation has been used to study response to high and low LET radiation. End points are tumor volume response, TCD50/180, clonogenic capability after tumour irradiation in situ, and in vitro cell survival after irradiation in both the well-oxygenated and the hypoxic conditions. Experience has shown that sublines with different growth rates, radiosensitivity and plating efficiency can arise from the same frozen stock. The conclusions that can be drawn from an analysis of the data obtained to date are as follows: 1. There is no correlation between the doubling times of our two cell lines growing in culture and in the animal. 2. RBE values obtained from growth delay and from TCD50 end points are in fair agreement. 3. Cell survival curves obtained after in situ irradiation show the cells to be more radioresistant than cells irradiated in suspension in vitro. 4. RBE values for cell survival after in situ irradiation compare favourably with those obtained both for tumour growth delay and in vitro cell survival. 5. Not enough is known at present about radiation-altered cell kinetics to develop a self-consistent model of tumour response after irradiation.
一只WAG/Rij大鼠患有横纹肌肉瘤，在肿瘤切除和酶解后具有集落生长能力，已被用于研究对高传能线密度和低传能线密度辐射的反应。观察终点包括肿瘤体积反应、TCD50/180、原位肿瘤照射后的克隆形成能力，以及在富氧和缺氧条件下照射后的体外细胞存活情况。经验表明，具有不同生长速率、放射敏感性和接种效率的亚系可能源自同一冻存株。根据对迄今获得的数据进行分析可得出以下结论：1. 我们的两个细胞系在体外培养和在动物体内生长时的倍增时间之间没有相关性。2. 从生长延迟和TCD50观察终点获得的相对生物学效应（RBE）值相当一致。3. 原位照射后获得的细胞存活曲线显示，这些细胞比体外悬浮照射的细胞对辐射更具抗性。4. 原位照射后细胞存活的RBE值与肿瘤生长延迟和体外细胞存活所获得的RBE值相比具有优势。5. 目前对于辐射改变的细胞动力学了解不足，无法建立一个关于照射后肿瘤反应的自洽模型。
