Kubilus J, Baden H P, McGilvray N
J Natl Cancer Inst. 1980 Nov;65(5):869-75.
The sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) pattern of the citrate-soluble fibrous protein isolated from human basal cell epitheliomas showed polypeptides with molecular weights of 43,000, 45,000, and 59,000. These polypeptides, found in the protein of normal epidermis, were present in a different proportion in this tumor tissue. Basal cell epitheliomas were cultured by the use of killed murine 3T3 feeder layers but only when 10(-9) M cholera toxin was present. The SDS-PAGE pattern of fibrous protein from cultured tumor cells was identical to the pattern of cultured normal human epidermal cells, and cornified cell envelopes were found in both types of cultures. Electron microscopy showed stratified cells that contained desmosomes, tonofilaments, and keratohyalin granules.
从人基底细胞上皮瘤中分离出的柠檬酸盐可溶性纤维蛋白的十二烷基硫酸钠（SDS）聚丙烯酰胺凝胶电泳（PAGE）图谱显示，其含有分子量分别为43,000、45,000和59,000的多肽。这些在正常表皮蛋白质中发现的多肽，在该肿瘤组织中的比例有所不同。通过使用灭活的鼠3T3饲养层培养基底细胞上皮瘤，但仅在存在10^(-9) M霍乱毒素时才能培养成功。培养的肿瘤细胞中纤维蛋白的SDS-PAGE图谱与培养的正常人表皮细胞的图谱相同，并且在两种培养物中均发现了角质化细胞包膜。电子显微镜显示，分层细胞含有桥粒、张力丝和透明角质颗粒。
