Breitkreutz D, Tilgen W, Boukamp P, Fusenig N E
Anticancer Res. 1981;1(6):323-8.
Prekeratin was reduced in human skin malignancies comparing Bowen's carcinoma (BC), basal cell carcinomas (BCC) and squamous cell carcinomas (SCC) with normal epidermis. This observation correlated with ultrastructural appearance and frequency of tonofilaments. Gel electrophoresis of tumor extracts revealed the decrease or loss of larger prekeratin peptides (65 to 68 K daltons) prominent in the epidermis. Biopsies, particularly from BC, resembled normal keratinocytes in culture with respect to their prekeratin patterns (48 to 61 K daltons). The pattern was most different, and prekeratin lowest, in SCC and derived cultures. In BCC-cultures, however, prekeratin (almost identical to keratinocytes) and filament formation significantly exceeded the respective tumor levels.
与正常表皮相比，人类皮肤恶性肿瘤（包括鲍恩病（BC）、基底细胞癌（BCC）和鳞状细胞癌（SCC））中的前角蛋白减少。这一观察结果与张力丝的超微结构外观和频率相关。肿瘤提取物的凝胶电泳显示，表皮中突出的较大前角蛋白肽（65至68千道尔顿）减少或缺失。活检样本，尤其是来自BC的样本，其前角蛋白模式（48至61千道尔顿）与培养中的正常角质形成细胞相似。SCC及其衍生培养物中的模式差异最大，前角蛋白含量最低。然而，在BCC培养物中，前角蛋白（几乎与角质形成细胞相同）和丝状结构的形成明显超过了各自肿瘤中的水平。
