Ketis N V, Girdlestone J, Grant C W
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3788-90. doi: 10.1073/pnas.77.7.3788.
A transmembrane glycoprotein, glycophorin, was assembled into large liposomes that may be handled like intact cells. Under appropriate conditions, lectin binding to these simple model cells accurately mimics the positive cooperative behavior commonly reported for binding of various lectins to real cells. Hence we suggest that the simple observation of cooperativity in such a cell-surface recognition event does not necessarily imply an involvement of complex cellular machinery; rather, it may simply reflect interaction of a multivalent ligand with conformationally deformable headgroups.
一种跨膜糖蛋白,血型糖蛋白,被组装到大型脂质体中,这些脂质体可以像完整细胞一样进行处理。在适当条件下,凝集素与这些简单模型细胞的结合准确地模拟了各种凝集素与真实细胞结合时常见的正协同行为。因此,我们认为在这种细胞表面识别事件中对协同性的简单观察不一定意味着涉及复杂的细胞机制;相反,它可能仅仅反映了多价配体与构象可变形头部基团的相互作用。