Engström C, Röckert H O
Swed Dent J. 1980;4(3):119-23.
Pulps from rat incisor have been dissected out and the respiratory activity in the tissues has been measured in a Gilson respirometer (modified Warburg technique). Local anesthetics lidocain (xylocain) and prilocain (citanest) in concentrations 2.5%, 5%, and 10% have been added to the medium. Effects on the membrane permeability i.e. on the potassium-sodium pump has been studied using incubation at 37 degrees C in 24NaCl. The influx of 24Na has been registered in a scintillation counter. The anaerobic respiratory activity has been studied determining lactate dehydrogenase activity with a spectrophotometric technique. The results show that the concentrations of local anesthetics in the medium of 5% and 10% caused a significantly lower respiratory activity of the cells in the dental pulp. This was not the case in the concentration 2.5%. The isotope studies show a decreased uptake in all concentrations indicating a depression of the potassium-sodium pump activity. The lactate dehydrogenase activity was significantly lower in all concentrations of local anesthetics.
已从大鼠切牙中取出牙髓，并在吉尔森呼吸计（改良的瓦伯格技术）中测量了组织中的呼吸活性。将浓度为2.5%、5%和10%的局部麻醉剂利多卡因（赛罗卡因）和丙胺卡因（卡替诺）添加到培养基中。使用在37℃下于24NaCl中孵育的方法，研究了对膜通透性即对钠钾泵的影响。通过闪烁计数器记录了24Na的流入量。使用分光光度技术通过测定乳酸脱氢酶活性研究了无氧呼吸活性。结果表明，培养基中5%和10%浓度的局部麻醉剂导致牙髓中细胞的呼吸活性显著降低。2.5%的浓度则并非如此。同位素研究表明，所有浓度下的摄取量均降低，表明钠钾泵活性受到抑制。在所有局部麻醉剂浓度下，乳酸脱氢酶活性均显著降低。
