Leite Mariana Ferreira, Ganzerla Emily, Marques Márcia Martins, Nicolau José
Centro de Pesquisa em Biologia Oral - Departamento de Biomateriais e Bioquímica Oral, School of Dentistry, Universidade de São Paulo, São Paulo, Brazil.
J Endod. 2008 Oct;34(10):1211-4. doi: 10.1016/j.joen.2008.07.010.
Diabetes can interfere in tissue nutrition and can impair dental pulp metabolism. This disease causes oxidative stress in cells and tissues. However, little is known about the antioxidant system in the dental pulp of diabetics. Thus, it would be of importance to study this system in this tissue in order to verify possible alterations indicative of oxidative stress. The aim of this study was to evaluate some parameters of antioxidant system of the dental pulp of healthy (n = 8) and diabetic rats (n = 8). Diabetes was induced by streptozotocin in rats. Six weeks after diabetes induction, a pool of the dental pulp of the 4 incisors of each rat (healthy and diabetic) was used for the determination of total protein and sialic acid concentrations and catalase and peroxidase activities. Data were compared by a Student t test (p <or= 0.05). Dental pulps from both groups presented similar total protein concentrations and peroxidase activity. Dental pulps of diabetic rats exhibited significantly lower free, conjugated, and total sialic acid concentrations than those of control tissues. Catalase activity in diabetic dental pulps was significantly enhanced in comparison with that of control pulps. The result of the present study is indicative of oxidative stress in the dental pulp caused by diabetes. The increase of catalase activity and the reduction of sialic acid could be resultant of reactive oxygen species production.
糖尿病会干扰组织营养并损害牙髓代谢。这种疾病会导致细胞和组织中的氧化应激。然而，对于糖尿病患者牙髓中的抗氧化系统知之甚少。因此，研究该组织中的这个系统以验证可能表明氧化应激的改变具有重要意义。本研究的目的是评估健康大鼠（n = 8）和糖尿病大鼠（n = 8）牙髓抗氧化系统的一些参数。通过链脲佐菌素诱导大鼠患糖尿病。糖尿病诱导六周后，将每组大鼠（健康和糖尿病）的4颗切牙的牙髓收集起来，用于测定总蛋白和唾液酸浓度以及过氧化氢酶和过氧化物酶活性。数据通过学生t检验进行比较（p≤0.05）。两组的牙髓总蛋白浓度和过氧化物酶活性相似。糖尿病大鼠的牙髓游离、结合和总唾液酸浓度显著低于对照组织。与对照牙髓相比，糖尿病牙髓中的过氧化氢酶活性显著增强。本研究结果表明糖尿病会导致牙髓氧化应激。过氧化氢酶活性增加和唾液酸减少可能是活性氧产生的结果。
