Weiner R S, Normann S J
J Natl Cancer Inst. 1981 Feb;66(2):255-60.
Human peripheral blood monocytes collected by semicontinuous flow centrifugation were cryopreserved in varying concentrations of dimethyl sulfoxide (DMSO). Optimum protection from lethal cryoinjury was achieved with 7.5-10% DMSO. Compared to fresh cells, cryopreserved cells retained 80% of their chemotactic responsiveness and fructose-6-phosphate shunt activity after stimulation by zymosan. Fresh and cryopreserved human monocytes had comparable but low levels of cytotoxic activity against P-815 murine mastocytoma cells in vitro.
通过半连续流动离心收集的人外周血单核细胞在不同浓度的二甲基亚砜（DMSO）中进行冷冻保存。使用7.5 - 10%的DMSO可实现对致死性冷冻损伤的最佳保护。与新鲜细胞相比，冷冻保存的细胞在经酵母聚糖刺激后保留了80%的趋化反应性和6 - 磷酸果糖分流活性。新鲜和冷冻保存的人单核细胞在体外对P - 815小鼠肥大细胞瘤细胞具有相当但较低水平的细胞毒活性。
