使用冷冻保存的正常外周血淋巴细胞从血清学阳性男性中分离人类免疫缺陷病毒。
Use of cryopreserved normal peripheral blood lymphocytes for isolation of human immunodeficiency virus from seropositive men.
作者信息
Balachandran R, Thampatty P, Rinaldo C, Gupta P
机构信息
Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pennsylvania 15261.
出版信息
J Clin Microbiol. 1988 Mar;26(3):595-7. doi: 10.1128/jcm.26.3.595-597.1988.
Abstract
The possibility was investigated of using frozen stocks of phytohemagglutinin (PHA)-stimulated normal human peripheral blood lymphocytes (PBL) in cocultivation with human immunodeficiency virus (HIV)-infected lymphocytes for the isolation of HIV. Fresh and cryopreserved PBL from eight healthy volunteers were compared for their susceptibility to HIV infection in vitro. Fresh lymphocytes, as well as lymphocytes that were stimulated with PHA before or after cryopreservation, displayed comparable susceptibilities to HIV infection in vitro. In addition, HIV was recovered in all cases when lymphocytes stimulated with PHA before or after cryopreservation were cocultured in parallel with PBL from 15 patients with acquired immune deficiency syndrome. However, the cryopreserved PBL were less efficient in isolating HIV from asymptomatic men.
摘要
研究了使用冷冻保存的植物血凝素（PHA）刺激的正常人外周血淋巴细胞（PBL）与人类免疫缺陷病毒（HIV）感染的淋巴细胞共培养以分离HIV的可能性。比较了来自8名健康志愿者的新鲜和冷冻保存的PBL在体外对HIV感染的易感性。新鲜淋巴细胞以及在冷冻保存之前或之后用PHA刺激的淋巴细胞在体外对HIV感染表现出相当的易感性。此外，当将冷冻保存之前或之后用PHA刺激的淋巴细胞与15例获得性免疫缺陷综合征患者的PBL平行共培养时，在所有情况下都能回收HIV。然而，冷冻保存的PBL从无症状男性中分离HIV的效率较低。
相似文献
1
Use of cryopreserved normal peripheral blood lymphocytes for isolation of human immunodeficiency virus from seropositive men.使用冷冻保存的正常外周血淋巴细胞从血清学阳性男性中分离人类免疫缺陷病毒。
J Clin Microbiol. 1988 Mar;26(3):595-7. doi: 10.1128/jcm.26.3.595-597.1988.
2
Isolation of human immunodeficiency virus from peripheral blood lymphocytes stored in various transport media and frozen at -60 degrees C.从储存在各种运输介质中并在-60℃下冷冻的外周血淋巴细胞中分离人类免疫缺陷病毒。
J Clin Microbiol. 1989 Jan;27(1):88-90. doi: 10.1128/jcm.27.1.88-90.1989.
3
Comparative studies on use of fresh and frozen peripheral blood lymphocyte specimens for isolation of human immunodeficiency virus and effects of cell lysis on isolation efficiency.新鲜和冷冻外周血淋巴细胞标本用于分离人类免疫缺陷病毒的比较研究及细胞裂解对分离效率的影响。
J Clin Microbiol. 1987 Jul;25(7):1291-4. doi: 10.1128/jcm.25.7.1291-1294.1987.
4
HIV-induced immunodeficiency. Relatively preserved phytohemagglutinin as opposed to decreased pokeweed mitogen responses may be due to possibly preserved responses via CD2/phytohemagglutinin pathway.人类免疫缺陷病毒（HIV）引起的免疫缺陷。与美洲商陆有丝分裂原反应降低相反，植物血凝素反应相对保留，这可能是由于通过CD2/植物血凝素途径的反应可能得以保留。
J Immunol. 1989 Mar 15;142(6):1874-80.
5
Isolation of HIV from Israeli patients and asymptomatic carriers.从以色列患者和无症状携带者中分离出艾滋病毒。
Isr J Med Sci. 1988 Mar;24(3):141-4.
6
Cell-to-cell transmission of human immunodeficiency virus type 1 in the presence of azidothymidine and neutralizing antibody.在齐多夫定和中和抗体存在的情况下，人类免疫缺陷病毒1型的细胞间传播
J Virol. 1989 May;63(5):2361-5. doi: 10.1128/JVI.63.5.2361-2365.1989.
7
8
Use of a new CD4-positive HeLa cell clone for direct quantitation of infectious human immunodeficiency virus from blood cells of AIDS patients.使用一种新的CD4阳性海拉细胞克隆直接定量艾滋病患者血细胞中的传染性人类免疫缺陷病毒。
J Infect Dis. 1991 Jan;163(1):64-70. doi: 10.1093/infdis/163.1.64.
9
The effect of fresh lymphocytes on increased sensitivity of HIV-1 isolation: a multicenter study.新鲜淋巴细胞对提高HIV-1分离敏感性的影响：一项多中心研究。
J Acquir Immune Defic Syndr (1988). 1990;3(10):981-6.
10
Optimal conditions for recovery of the human immunodeficiency virus from peripheral blood mononuclear cells.从外周血单核细胞中分离人类免疫缺陷病毒的最佳条件。
J Clin Microbiol. 1988 Nov;26(11):2371-6. doi: 10.1128/jcm.26.11.2371-2376.1988.
引用本文的文献
1
Advances in CAR T cell therapy: antigen selection, modifications, and current trials for solid tumors.嵌合抗原受体T细胞疗法的进展：抗原选择、修饰及实体瘤的当前试验
Front Immunol. 2025 Jan 6;15:1489827. doi: 10.3389/fimmu.2024.1489827. eCollection 2024.
2
Detection of replication-competent human immunodeficiency virus type 1 (HIV-1) in cultures from patients with levels of HIV-1 RNA in plasma suppressed to less than 500 or 50 copies per milliliter.在血浆中HIV-1 RNA水平被抑制至低于每毫升500或50拷贝的患者的培养物中检测具有复制能力的1型人类免疫缺陷病毒（HIV-1）。
J Clin Microbiol. 2002 Jun;40(6):2089-94. doi: 10.1128/JCM.40.6.2089-2094.2002.
3
Immunomagnetic selection of purified monocyte and lymphocyte populations from peripheral blood mononuclear cells following cryopreservation.冷冻保存后从外周血单个核细胞中进行免疫磁选纯化单核细胞和淋巴细胞群体。
Clin Diagn Lab Immunol. 1997 Nov;4(6):653-8. doi: 10.1128/cdli.4.6.653-658.1997.
4
Effective use of frozen donor peripheral blood mononuclear cells for human immunodeficiency virus type 1 isolation from vertically infected pediatric patients.有效利用冷冻供体外周血单个核细胞从垂直感染的儿科患者中分离1型人类免疫缺陷病毒
J Clin Microbiol. 1994 May;32(5):1379-82. doi: 10.1128/jcm.32.5.1379-1382.1994.
5
Cell-to-cell transmission of human immunodeficiency virus type 1 in the presence of azidothymidine and neutralizing antibody.在齐多夫定和中和抗体存在的情况下，人类免疫缺陷病毒1型的细胞间传播
J Virol. 1989 May;63(5):2361-5. doi: 10.1128/JVI.63.5.2361-2365.1989.
6
The diagnosis of human immunodeficiency virus infection: progress in less than five years.人类免疫缺陷病毒感染的诊断：五年内的进展
Yale J Biol Med. 1989 Mar-Apr;62(2):93-102.
7
Prospective multicenter study on isolation of human immunodeficiency virus type 1 from homosexual men after seroconversion.血清转化后从同性恋男性中分离出1型人类免疫缺陷病毒的前瞻性多中心研究。
J Clin Microbiol. 1991 Jul;29(7):1368-71. doi: 10.1128/jcm.29.7.1368-1371.1991.
8
Standardization of sensitive human immunodeficiency virus coculture procedures and establishment of a multicenter quality assurance program for the AIDS Clinical Trials Group. The NIH/NIAID/DAIDS/ACTG Virology Laboratories.人类免疫缺陷病毒敏感共培养程序的标准化以及艾滋病临床试验组多中心质量保证项目的建立。美国国立卫生研究院/美国国立过敏与传染病研究所/艾滋病司/艾滋病临床试验组病毒学实验室。
J Clin Microbiol. 1992 Jul;30(7):1787-94. doi: 10.1128/jcm.30.7.1787-1794.1992.
本文引用的文献
1
Functional integrity of cryopreserved human monocytes.冷冻保存的人单核细胞的功能完整性
J Natl Cancer Inst. 1981 Feb;66(2):255-60.
2
Lymphadenopathy associated virus infection of a blood donor--recipient pair with acquired immunodeficiency syndrome.一名艾滋病病毒感染者献血者与其受血者之间的淋巴结病相关病毒感染
Science. 1984 Jul 6;225(4657):69-72. doi: 10.1126/science.6328663.
3
Gene transfer, expression, and molecular cloning of the human transferrin receptor gene.人转铁蛋白受体基因的基因转移、表达及分子克隆
Cell. 1984 May;37(1):95-103. doi: 10.1016/0092-8674(84)90304-0.
4
Transferrin receptor induction in mitogen-stimulated human T lymphocytes is required for DNA synthesis and cell division and is regulated by interleukin 2.丝裂原刺激的人T淋巴细胞中转铁蛋白受体的诱导对于DNA合成和细胞分裂是必需的，并且受白细胞介素2调节。
Proc Natl Acad Sci U S A. 1983 Jun;80(11):3494-8. doi: 10.1073/pnas.80.11.3494.
5
Isolation of lymphocytopathic retroviruses from San Francisco patients with AIDS.从旧金山艾滋病患者中分离淋巴细胞病变逆转录病毒。
Science. 1984 Aug 24;225(4664):840-2. doi: 10.1126/science.6206563.
6
Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS.从艾滋病患者和有患艾滋病风险的人群中频繁检测和分离出细胞病变逆转录病毒（HTLV-III）。
Science. 1984 May 4;224(4648):500-3. doi: 10.1126/science.6200936.
7
Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS.从艾滋病患者和艾滋病前期患者中检测、分离并持续生产细胞病变逆转录病毒（HTLV-III）。
Science. 1984 May 4;224(4648):497-500. doi: 10.1126/science.6200935.
8
The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus.CD4（T4）抗原是艾滋病逆转录病毒受体的重要组成部分。
Nature. 1984;312(5996):763-7. doi: 10.1038/312763a0.
9
HTLV-III in saliva of people with AIDS-related complex and healthy homosexual men at risk for AIDS.艾滋病相关综合征患者及有艾滋病风险的健康同性恋男性唾液中的人类嗜T淋巴细胞病毒III型
Science. 1984 Oct 26;226(4673):447-9. doi: 10.1126/science.6093247.
10
Molecular cloning and expression of cDNAs for the human interleukin-2 receptor.人白细胞介素-2受体cDNA的分子克隆与表达
Nature. 1984;311(5987):626-31. doi: 10.1038/311626a0.
Zotero 插件