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一种用于膜生物化学的非变性两性离子去污剂：设计与合成。

A nondenaturing zwitterionic detergent for membrane biochemistry: design and synthesis.

作者信息

Hjelmeland L M

出版信息

Proc Natl Acad Sci U S A. 1980 Nov;77(11):6368-70. doi: 10.1073/pnas.77.11.6368.

DOI:10.1073/pnas.77.11.6368
PMID:6935651
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC350285/
Abstract

The synthesis and evaluation of a new detergent that is a zwitterionic derivative of cholic acid is presented. This detergent combines the useful properties of both the sulfobetaine-type detergents and the bile salt anions. The new detergent proved to be effective at solubilizing membrane proteins in a nondenatured state.

摘要

本文介绍了一种新型去污剂的合成与评估，该去污剂是胆酸的两性离子衍生物。这种去污剂兼具磺基甜菜碱型去污剂和胆盐阴离子的有用特性。新去污剂在溶解非变性状态的膜蛋白方面被证明是有效的。

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本文引用的文献

1
Solubilization of active opiate receptors.活性阿片受体的增溶作用。
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4623-7. doi: 10.1073/pnas.77.8.4623.
2
The fluid mosaic model of the structure of cell membranes.细胞膜结构的流动镶嵌模型。
Science. 1972 Feb 18;175(4023):720-31. doi: 10.1126/science.175.4023.720.
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Detergent extraction of erythrocyte ghosts. Comparison of residues after cholate and Triton X-100 treatments.
Biochim Biophys Acta. 1976 Jun 4;436(1):38-44. doi: 10.1016/0005-2736(76)90217-0.
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Solubilization of membranes by detergents.用去污剂溶解细胞膜。
Biochim Biophys Acta. 1975 Mar 25;415(1):29-79. doi: 10.1016/0304-4157(75)90016-7.
5
Effects of different bile salts upon the composition and morphology of a liver plasma membrane preparation. Deoxycholate is more membrane damaging than cholate and its conjugates.不同胆汁盐对肝细胞膜制剂的组成和形态的影响。脱氧胆酸盐比胆酸盐及其共轭物对膜的损伤更大。
Biochim Biophys Acta. 1977 Feb 14;465(1):68-76. doi: 10.1016/0005-2736(77)90356-x.
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Solubilization of membrane proteins by sulfobetaines, novel zwitterionic surfactants.
Anal Biochem. 1978 Jun 15;87(1):28-38. doi: 10.1016/0003-2697(78)90565-1.
7
Electrofocusing of integral membrane proteins in mixtures of zwitterionic and nonionic detergents.两性离子和非离子去污剂混合物中整合膜蛋白的等电聚焦
Anal Biochem. 1979 May;95(1):201-8. doi: 10.1016/0003-2697(79)90206-9.
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Properties of detergents.洗涤剂的特性。
Methods Enzymol. 1979;56:734-49. doi: 10.1016/0076-6879(79)56066-2.
9
Characterization of membrane proteins in detergent solutions.
Biochim Biophys Acta. 1976 Oct 26;457(2):133-70. doi: 10.1016/0304-4157(76)90009-5.