一种用于膜生物化学的非变性两性离子去污剂：设计与合成。
A nondenaturing zwitterionic detergent for membrane biochemistry: design and synthesis.
作者信息
Hjelmeland L M
出版信息
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6368-70. doi: 10.1073/pnas.77.11.6368.
Abstract
The synthesis and evaluation of a new detergent that is a zwitterionic derivative of cholic acid is presented. This detergent combines the useful properties of both the sulfobetaine-type detergents and the bile salt anions. The new detergent proved to be effective at solubilizing membrane proteins in a nondenatured state.
摘要
本文介绍了一种新型去污剂的合成与评估，该去污剂是胆酸的两性离子衍生物。这种去污剂兼具磺基甜菜碱型去污剂和胆盐阴离子的有用特性。新去污剂在溶解非变性状态的膜蛋白方面被证明是有效的。
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本文引用的文献
1
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Proc Natl Acad Sci U S A. 1980 Aug;77(8):4623-7. doi: 10.1073/pnas.77.8.4623.
2
The fluid mosaic model of the structure of cell membranes.细胞膜结构的流动镶嵌模型。
Science. 1972 Feb 18;175(4023):720-31. doi: 10.1126/science.175.4023.720.
3
Detergent extraction of erythrocyte ghosts. Comparison of residues after cholate and Triton X-100 treatments.
Biochim Biophys Acta. 1976 Jun 4;436(1):38-44. doi: 10.1016/0005-2736(76)90217-0.
4
Solubilization of membranes by detergents.用去污剂溶解细胞膜。
Biochim Biophys Acta. 1975 Mar 25;415(1):29-79. doi: 10.1016/0304-4157(75)90016-7.
5
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6
Solubilization of membrane proteins by sulfobetaines, novel zwitterionic surfactants.
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