Byers H R, Fujiwara K, Porter K R
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6657-61. doi: 10.1073/pnas.77.11.6657.
Microtubule staining patterns can be visualized within cells in situ on the surface of fish scales from the squirrel fish, Holocentrus ascensionis, and the common goldfish, Carassius auratus, after incubation with antibodies to sea urchin tubulin and fluorescein-labeled goat antibodies to rabbit immunoglobulin G. Chromatophores in situ from both species reveal a radial microtubule framework that orients the alignment of pigment granules. Innervating fibers of erythrophores on the H. ascensionis scale can also be observed. In situ, pseudo-epithelial cells called scleroblasts show microtubule patterns with a remarkable degree of similarity within a selected region. Over 90% of the cells have a microtubule framework that is nearly superimposable from cell to adjacent cell. The microtubules in scleroblasts are few and form a simple radial framework with a localized microtubule organizing center (MTOC). Microtubules in scleroblasts in vitro emanate from localized MTOCs but are much less radially organized than in situ. Scleroblasts in situ on the scale of C. auratus show microtubules that curve abruptly into coalignment with phase striations on the fibrillary plate. The phase striations arise from the orthogonal plies of collagen in intimate association with the scleroblasts. The role of microtubules in scleroblasts may thus be to provide orientation for collagen fibrillogenesis, analogous to their role in orientation of cellulose fibers in plants. That cells in situ exhibit highly related and coordinated microtubule staining patterns reaffirms that the cytoskeleton plays an important role in the organization of differentiated tissues.
在用抗海胆微管蛋白抗体和荧光素标记的山羊抗兔免疫球蛋白G抗体孵育后，松鼠鱼（Holocentrus ascensionis）和普通金鱼（Carassius auratus）鳞片表面细胞内的微管染色模式可以在原位观察到。两种鱼的色素细胞原位显示出一个径向微管框架，该框架使色素颗粒排列定向。在松鼠鱼鳞片上还可以观察到红色素细胞的神经支配纤维。原位观察时，称为成骨细胞的假上皮细胞在选定区域内显示出高度相似的微管模式。超过90%的细胞具有几乎从一个细胞到相邻细胞可重叠的微管框架。成骨细胞中的微管很少，形成一个简单的径向框架，有一个局部的微管组织中心（MTOC）。体外培养的成骨细胞中的微管从局部的MTOC发出，但径向排列程度远低于原位。金鱼鳞片上的原位成骨细胞显示微管突然弯曲，与纤维板上的相条纹对齐。相条纹由与成骨细胞紧密相连的正交胶原层产生。因此，微管在成骨细胞中的作用可能是为胶原纤维生成提供方向，类似于它们在植物纤维素纤维定向中的作用。原位细胞表现出高度相关和协调的微管染色模式，再次证明细胞骨架在分化组织的组织中起重要作用。
