Hørsted P, Fejerskov O, Johannesen G
Scand J Dent Res. 1980 Oct;88(5):412-7. doi: 10.1111/j.1600-0722.1980.tb01247.x.
In 35 roots from extracted human teeth the pulp tissue was removed and the specimens autoclaved before being transplanted subcutaneously to rats. The animals were sacrificed 7, 14, 21, 90 and 140 d after transplantation and the skin and subcutaneous tissue containing the transplants were prepared for histologic examination. The human roots were well tolerated by the rats with a fibrillar connective tissue capsule surrounding the specimens. In the 90-d and 14-d groups a few root surfaces exhibited small resorption lacunae. The empty root canals became slowly occupied by a maturing granulation tissue occasionally containing foci of lymphocytes. After 140 d the canal tissue was still highly vascular with multinuclear giant cells occasionally located along the dentin walls but no internal resorption was recorded. It is concluded that this model may be useful for systematic testing of root filling materials concomitant with human studies.
从拔除的人牙中获取35颗牙根，去除牙髓组织，将标本高压灭菌后皮下移植到大鼠体内。在移植后7、14、21、90和140天处死动物，制备包含移植物的皮肤和皮下组织用于组织学检查。大鼠对人牙根耐受性良好，标本周围有纤维结缔组织包膜。在90天和14天组中，一些牙根表面出现小的吸收腔隙。空的根管逐渐被成熟的肉芽组织占据，偶尔含有淋巴细胞灶。140天后，根管组织仍然血管丰富，偶尔有沿牙本质壁分布的多核巨细胞，但未记录到内部吸收。结论是该模型可能有助于在人体研究的同时对根管充填材料进行系统测试。
