Salminen S O
Biochim Biophys Acta. 1981 Mar 13;658(1):1-9. doi: 10.1016/0005-2744(81)90243-6.
Nodule breis and bacteroid preparations were made from Pisum sativum L. (cv. Trapper) inoculated with a single strain of Rhizobium leguminosarum. The detached nodules were triturated under helium flow. The resultant breis could support C2H2 reduction in N-Tris[hydroxymethyl]methyl-2-aminoethane-sulfonic acid buffer (Tes) without any additions for over an hour. NH4+ was found to inhibit C2H2 reduction and H2 evolution. The inhibition was not dependent on the counterion and was evident immediately after the addition of NH4+ to the reaction mixture. L-Methionine-D,L-sulfoximine, added to inhibit assimilation of NH4+, had no effect on the inhibition. Addition of pyruvate enhanced the rate of C2H2 reduction in breis and partially overcame the inhibition of NH4+. Pyruvate was found necessary for measurable activity in bacteroid preparations. When ATP and an ATP-generating system were used in breis the effect of NH4+ was not observed.
从接种了单一菌株的豌豆根瘤菌的豌豆（品种为Trapper）中制备根瘤匀浆和类菌体制剂。将分离的根瘤在氦气流下研磨。所得匀浆在N-三[羟甲基]甲基-2-氨基乙烷磺酸缓冲液（Tes）中无需添加任何物质就能支持乙炔还原超过一小时。发现铵离子会抑制乙炔还原和氢气释放。这种抑制不依赖于抗衡离子，并且在向反应混合物中添加铵离子后立即显现。添加L-蛋氨酸-D,L-亚砜亚胺以抑制铵离子的同化作用，对这种抑制没有影响。添加丙酮酸可提高匀浆中乙炔还原的速率，并部分克服铵离子的抑制作用。发现丙酮酸是类菌体制剂中可测量活性所必需的。当在匀浆中使用ATP和ATP生成系统时，未观察到铵离子的影响。
