Wakeland E K, Klein J
J Immunol. 1981 May;126(5):1731-6.
The I-region products of 13 congenic B10.W lines carrying wild-derived H-2 haplotypes were analyzed with anti-B10.W alloantisera. The alloantisera were tested for immunoprecipitating activity with radiolabeled glycoproteins of the immunizing donors. Eleven of the 13 alloantisera immunoprecipitated glycoproteins with SDS-PAGE mobilities consistent with m.w. of 45,000 35,000, and 26,000. The 35,000 and 26,000 products were identified as the Ia antigens of the B10.W lines. The specificity of the anti-Ia antibodies in each anti-B10.W alloantiserum was assessed by panel testing and absorption analysis with a panel of 26 glycoprotein fractions that were prepared from 11 inbred lines and 15 B10.W lines. Four new Ia antigens that are only present in the B10.W lines were detected in this analysis. The I-region products of 8 of the B10.W lines were serologically indistinguishable from those found among laboratory inbred strains. The implications of these findings for I-region polymorphisms are discussed.
利用抗B10.W同种抗血清分析了携带野生型H-2单倍型的13个同源B10.W系的I区产物。用免疫供体的放射性标记糖蛋白检测了同种抗血清的免疫沉淀活性。13种同种抗血清中的11种免疫沉淀了糖蛋白，其SDS-PAGE迁移率与分子量45,000、35,000和26,000一致。35,000和26,000的产物被鉴定为B10.W系的Ia抗原。通过用由11个近交系和15个B10.W系制备的26种糖蛋白组分进行分组检测和吸收分析，评估了每种抗B10.W同种抗血清中抗Ia抗体的特异性。在该分析中检测到4种仅存在于B10.W系中的新Ia抗原。8个B10.W系的I区产物在血清学上与实验室近交系中发现的产物无法区分。讨论了这些发现对I区多态性的影响。
