Welker D L, Williams K L
J Gen Microbiol. 1980 Sep;120(1):149-59. doi: 10.1099/00221287-120-1-149.
The assignment to linkage group VII of the coumarin sensitivity mutation couA351, which leads to a loss of colony-forming ability on agar containing coumarin, was established using the linkage group VII markers cobA1 and tsgK21. Complementation of the couA351 and bsgA5 mutations was an effective method of selecting heterozygous diploids at 21 +/- 1 degree C, i.e. without requiring a temperature sensitivity mutation. A morphological mutation, frtB353, which affects the distribution of fruiting bodies was also assigned to linkage group VII. Both the couA351 and frtB353 mutations were discovered in the tsgK21 strain NP187. Eight independently isolated, recessive mutations leading to resistance of 300 micrograms CoCl2 ml-1 were allelic with the cobA1 mutation. The partially dominant cob-353 mutation was shown to map in linkage group VII on the basis of its segregation relative to the couA351 and tsgK21 mutations and is almost certainly an allele of the cobA locus. These results are consistent with there being only a single locus at which mutations can lead to resistance to high concentrations of CoCl2. Two temperature sensitivity mutations tsgM357 and tsgG4 were also assigned to linkage group VII.
香豆素敏感性突变体couA351被定位到连锁群VII，该突变导致在含有香豆素的琼脂上丧失菌落形成能力，这是利用连锁群VII的标记cobA1和tsgK21确定的。在21±1℃下，couA351和bsgA5突变体的互补作用是选择杂合二倍体的有效方法，即无需温度敏感性突变。一种影响子实体分布的形态突变体frtB353也被定位到连锁群VII。couA351和frtB353突变均在tsgK21菌株NP187中被发现。八个独立分离的、导致对300微克/毫升CoCl2产生抗性的隐性突变与cobA1突变等位。基于其相对于couA351和tsgK21突变的分离情况，部分显性的cob - 353突变被证明定位于连锁群VII，几乎可以肯定它是cobA位点的一个等位基因。这些结果与只有一个位点的突变可导致对高浓度CoCl2产生抗性这一情况相符。两个温度敏感性突变体tsgM357和tsgG4也被定位到连锁群VII。
