使用多重标记测试菌株对盘基网柄菌进行连锁分析：VII连锁群的建立及对早期连锁数据的重新评估。

Linkage analysis in Dictyostelium discoideum using multiply marked tester strains: establishment of linkage group VII and the reassessment of earlier linkage data.

作者信息

Ratner D I, Newell P C

出版信息

J Gen Microbiol. 1978 Dec;109(2):225-36. doi: 10.1099/00221287-109-2-225.

Abstract

To aid linkage analysis and mapping studies in Dictyostelium discoideum, we have constructed several tester strains with easily scored mutations characterizing the six currently identified linkage groups. Use has been made of conditionally lethal mutants unable to grow upon Bacillus subtilis, and the locus of the mutation involved (bsgA) has been assigned to linkage group III. The mutation cobA1, which confers resistance to cobaltous chloride, has been assigned to a previously unidentified linkage group (VII). The temperature-sensitive growth mutation tsgC7, previously reported to define linkage group V, has been reassigned to group III, leaving linkage group V presently unmarked. The further use of genetic tester strains is described.

摘要

为了辅助盘基网柄菌的连锁分析和图谱研究，我们构建了几个测试菌株，这些菌株带有易于评分的突变，可表征目前已鉴定的六个连锁群。我们利用了在枯草芽孢杆菌上无法生长的条件致死突变体，并且已将所涉及的突变位点（bsgA）定位到连锁群III。赋予对氯化钴抗性的突变cobA1已被定位到一个先前未鉴定的连锁群（VII）。先前报道用于定义连锁群V的温度敏感生长突变tsgC7已被重新定位到群III，使得连锁群V目前没有标记。本文还描述了遗传测试菌株的进一步应用。