Gross L, Dreyfuss Y, Ehrenreich T, Feldman D, Limbert L M
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7435-9. doi: 10.1073/pnas.77.12.7435.
In vitro gamma irradiation of virus-induced (Gross) mouse leukemia cells at doses of 350-1600 rads (1 rad = 0.01 gray) had no effect on their ability to induce leukemia, usually within 2 weeks, after transplantation into syngeneic mice. However, when cells irradiated at doses of 2000-20,000 rads were transplanted, they induced leukemia after a latency period exceeding 2.5 months, similar to the results observed in mice inoculated with filtered mouse leukemia extracts. Similar results were also obtained after irradiation of leukemic cells derived from rats in which leukemia had been induced by rat-adapted mouse leukemia virus. Apparently, gamma irradiation at a dose of, or exceeding, 2000 rads, inhibits the ability of mouse and rat leukemic cells to induce leukemia after transplantation into syngeneic hosts; however, it does not inactivate the virus carried by such cells nor prevent it from inducing leukemia. [In previous experiments, doses of more than 4,500,000 rads were needed to inactivate the passage A (Gross) leukemia virus carried in either mouse or rat leukemic cells.] In vitro gamma irradiation of L2C guinea pig leukemic cells at doses of 750-2500 rads had no apparent effect on their ability to induce leukemia after transplantation effect on their ability to induce leukemia after transplantation into strain 2 guinea pigs. However, irradiation at doses of 3250-20,000 rads inactivated their ability to do so. The morphology of mouse, rat, and guinea pig leukemic cells and the virus particles present in such cells was not affected by irradiation at doses of 20,000 rads.
用350 - 1600拉德（1拉德 = 0.01戈瑞）的剂量对病毒诱导的（格罗斯）小鼠白血病细胞进行体外γ射线照射，对其在移植到同基因小鼠体内后通常在2周内诱发白血病的能力没有影响。然而，当移植经2000 - 20000拉德剂量照射的细胞时，它们在超过2.5个月的潜伏期后诱发白血病，这与接种过滤后的小鼠白血病提取物的小鼠中观察到的结果相似。在用大鼠适应的小鼠白血病病毒诱发白血病的大鼠来源的白血病细胞照射后，也得到了类似的结果。显然，2000拉德及以上剂量的γ射线照射会抑制小鼠和大鼠白血病细胞移植到同基因宿主后诱发白血病的能力；然而，它不会使此类细胞携带的病毒失活，也不会阻止其诱发白血病。[在先前的实验中，需要超过4500000拉德的剂量才能使小鼠或大鼠白血病细胞中携带的A株（格罗斯）白血病病毒失活。]用750 - 2500拉德的剂量对L2C豚鼠白血病细胞进行体外γ射线照射，对其移植到2系豚鼠后诱发白血病的能力没有明显影响。然而，3250 - 20000拉德剂量的照射使其失去了这种能力。20000拉德剂量的照射不会影响小鼠、大鼠和豚鼠白血病细胞的形态以及此类细胞中存在的病毒颗粒。
