牛γ球蛋白与L-天冬酰胺酶（大肠杆菌）的偶联（作者译）
[Conjugation of bovine gammaglobulin to L-asparaginase (E. coli) (author's transl)].
作者信息
Hädge D, Ambrosius H
出版信息
Allerg Immunol (Leipz). 1977;23(1):17-23.
PMID:69393
Abstract
Bovine gammaglobulin-L-asparaginase conjugates were prepared with an enzymic activity of about 30 IU/mg. Glutardialdehyde was used as bifunctional reagent for the cross-linking in an optimal concentration of 0.16 mg/10 mg protein. The both components were found in the conjugates in a ratio of 1:1.5. The recovery was about 25% of the initial protein amounts. The antigenic specificity of the globulin component decreased up to 60%, the enzymic activity also up to 60% by the conjugation.
摘要
制备了具有约30 IU/mg酶活性的牛γ球蛋白-L-天冬酰胺酶缀合物。戊二醛用作双功能试剂进行交联，最佳浓度为0.16 mg/10 mg蛋白质。在缀合物中发现两种成分的比例为1:1.5。回收率约为初始蛋白量的25%。通过缀合，球蛋白成分的抗原特异性降低高达60%，酶活性也降低高达60%。
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