Dunlap R M, Gray J L, Turner D W, Vernino A R, Williams J E, Pelleu G B
J Periodontol. 1981 Mar;52(3):140-2. doi: 10.1902/jop.1981.52.3.140.
Controversy exists over the potential for new connective tissue attachment to dentin of endodontically-obturated teeth following periodontal therapy. The purpose of this study was to determine whether cultured human gingival fibroblasts would grow in vitro on planed dentin surfaces of endodontically-treated teeth. A model similar to that of Aleo et al. was developed using extracted endodontically-treated human teeth. This model consisted of longitudinally sectioning 10 teeth, root planing one section only, and incubating both sections with a suspension of human gingival fibroblasts. Fibroblast growth was determined by staining with neutral red and trypan blue. The criterion for growth was staining of the complete root surface. All root areas with attached periodontal fibers displayed staining, as well as the complete root surface of all planed sections. Unplaned sections did not stain on root areas formerly exposed to the oral environment owing to periodontal disease, a finding consistent with the results of Aleo et al. Our results indicate that root canal therapy does not interfere with in vitro growth of fibroblasts on planed dentin surfaces of endodontically-treated teeth. Extrapolation to a clinical situation would indicate that normal healing may be expected after periodontal surgery on tissues adjacent to root planed endodontically-treated teeth.
对于牙周治疗后新的结缔组织附着于根管充填牙本质的可能性存在争议。本研究的目的是确定培养的人牙龈成纤维细胞是否会在根管治疗牙齿的平面牙本质表面上进行体外生长。使用拔除的根管治疗人牙建立了一个类似于阿莱奥等人的模型。该模型包括将10颗牙齿纵向切片，仅对其中一个切片进行根面平整，并用人牙龈成纤维细胞悬液孵育两个切片。通过中性红和台盼蓝染色来确定成纤维细胞的生长。生长的标准是整个牙根表面染色。所有附着有牙周纤维的牙根区域均显示染色，以及所有平整切片的整个牙根表面。未平整的切片在因牙周病而先前暴露于口腔环境的牙根区域未染色，这一发现与阿莱奥等人的结果一致。我们的结果表明，根管治疗不会干扰成纤维细胞在根管治疗牙齿的平面牙本质表面上的体外生长。外推至临床情况表明，对根管治疗后进行根面平整的牙齿相邻组织进行牙周手术后，有望实现正常愈合。
