抗兔孕酮受体抗体：与人受体的交叉反应。
Antibodies to rabbit progesterone receptor: crossreaction with human receptor.
作者信息
Logeat F, Hai M T, Milgrom E
出版信息
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1426-30. doi: 10.1073/pnas.78.3.1426.
Abstract
Progesterone receptor from rabbit uterine cytosol was purified to a specific activity of approximately 2 nmol of bound hormone per mg of protein. A goat was immunized with this preparation and, after two injections of 0.7-0.8 nmol, yielded antireceptor antibodies. The antiserum reacted with both cytosolic and nuclear rabbit progesterone receptor and also with progesterone receptor from other rabbit tissues (vagina and pituitary). A crossreaction was observed with progesterone receptors from other mammalian, especially human, tissues (cytosolic receptor from rat and guinea pig uterus, cytosolic receptor from human breast cancer, and nuclear receptor from human endometrium). On the contrary, there was no interaction with a nonmammalian receptor (chicken oviduct progesterone receptor). The antibodies did not crossreact with other rabbit steroid receptors (uterine estradiol receptor and liver glucocorticoid receptor) or with nonreceptor progesterone-binding proteins (transcortin from plasma and uteroglobin from uterine fluid).
摘要
兔子宫胞质中的孕酮受体被纯化至每毫克蛋白质约2纳摩尔结合激素的比活性。用该制剂免疫一只山羊，在两次注射0.7 - 0.8纳摩尔后，产生了抗受体抗体。该抗血清与兔胞质和核孕酮受体反应，也与来自兔其他组织（阴道和垂体）的孕酮受体反应。观察到与来自其他哺乳动物，尤其是人类组织的孕酮受体有交叉反应（大鼠和豚鼠子宫的胞质受体、人乳腺癌的胞质受体以及人子宫内膜的核受体）。相反，与非哺乳动物受体（鸡输卵管孕酮受体）没有相互作用。这些抗体与兔其他类固醇受体（子宫雌二醇受体和肝脏糖皮质激素受体）或与非受体孕酮结合蛋白（血浆中的皮质素转运蛋白和子宫液中的子宫珠蛋白）没有交叉反应。
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