Tenovuo J, Mielityinen H, Larjava H, Paunio K
Acta Odontol Scand. 1980;38(6):355-61. doi: 10.3109/00016358009033605.
Lysosomal hydrolases as indicators of plaque-induced bone resorption in tissue culture were studied. Fetal rat bones cultured in a synthetic medium containing sonicated and filtrated human dental plaque were used as bones to be resorbed. Acid phosphatase and beta-glucuronidase were found to be the most suitable enzymes for studying the degradation rate of bone cell lysosomes. When dental plaque is used as a resorbing agent, special attention has to be paid to the fact that plaque extract contains similar lysosomal hydrolase activity. Plaque hydrolases in the present study were quite stable in culture medium. No significant adsorption of enzymes by fetal rat bones occurred while remarkable adsorption by synthetic hydroxyapatite was found. The present results indicate that dental plaque is able to release lysosomal hydrolases from bone explants. This release corresponds to the degree of resorption measured by 45Ca release from bones.
研究了溶酶体水解酶作为组织培养中菌斑诱导骨吸收指标的情况。将在含有经超声处理和过滤的人牙菌斑的合成培养基中培养的胎鼠骨骼用作待吸收的骨骼。发现酸性磷酸酶和β-葡萄糖醛酸酶是研究骨细胞溶酶体降解率最合适的酶。当使用牙菌斑作为吸收剂时，必须特别注意菌斑提取物含有类似溶酶体水解酶活性这一事实。本研究中的菌斑水解酶在培养基中相当稳定。胎鼠骨骼对酶没有明显吸附，而合成羟基磷灰石对酶有显著吸附。目前的结果表明，牙菌斑能够从骨外植体中释放溶酶体水解酶。这种释放与通过骨骼中45Ca释放测量的吸收程度相对应。
