Martínez-Almoyna C, Claver Criado M, Monereo González J, Contreras F
An Esp Pediatr. 1977 Apr;10(4):341-50.
Between March 1972 and Dec. 1974 532 rectal suction biopsies were obtained from 238 children and histochemical processed. Slight haemorrhage was observed in three. Indications were :1) Group A, 185 suspected aganglionism (AChE. and DPNH) activities were studied). 2) Group B, 7 Neurolipidoses (Red-oil-o) and 3) Group C, Post-operative control in 10 aganglionic patients (AChE, DPNH). Aganglionism was confirmed in 60 cases (25.2%) of Group A [AChE (+), DPNH (-)] although there were five false negatives (8.3%). Lipidosis was confirmed in one case of Group B. Four patients in Group C were AChE and DPNH (+). Results are discussed, and this technique recommended taking in mind its minimal morbidity, simplicity and good results.
1972年3月至1974年12月期间，从238名儿童身上获取了532份直肠吸引活检组织，并进行了组织化学处理。有3例出现轻微出血。适应证为：1）A组，185例疑似无神经节细胞症（研究乙酰胆碱酯酶和二磷酸烟酰胺腺嘌呤二核苷酸活性）。2）B组，7例神经脂质沉积症（红油-O），以及3）C组，10例无神经节细胞症患者术后对照（乙酰胆碱酯酶、二磷酸烟酰胺腺嘌呤二核苷酸）。A组60例（25.2%）确诊为无神经节细胞症【乙酰胆碱酯酶（+），二磷酸烟酰胺腺嘌呤二核苷酸（-）】，不过有5例假阴性（8.3%）。B组1例确诊为脂质沉积症。C组4例患者乙酰胆碱酯酶和二磷酸烟酰胺腺嘌呤二核苷酸呈阳性。对结果进行了讨论，并推荐采用该技术，因其发病率极低、操作简单且效果良好。
