先天性巨结肠的组织化学诊断以及直肠黏膜活检中乙酰胆碱酯酶组织化学与生化活性的比较
Histochemical diagnosis of Hirschsprung's disease and a comparison of the histochemical and biochemical activity of acetylcholinesterase in rectal mucosal biopsies.
作者信息
Patrick W J, Besley G T, Smith I I
出版信息
J Clin Pathol. 1980 Apr;33(4):336-43. doi: 10.1136/jcp.33.4.336.
Abstract
Three hundred and seventy-two rectal mucosal biopsies, taken from 150 children and young adults with chronic constipation, were subjected to histochemical and biochemical analysis of acetylcholinesterase to excude Hirschsprung's disease. The relative merits of the procedures were compared. The histochemical method was considered to be the most practical for laboratories handling small numbers of biopsies but the biochemical estimation of acetylcholinesterase activity was found to be a useful complementary procedure and an accurate quantitative assessment of enzyme activity.
摘要
对150名患有慢性便秘的儿童和年轻人进行直肠黏膜活检，共获取372份活检样本，对其进行乙酰胆碱酯酶的组织化学和生化分析，以排除先天性巨结肠症。比较了这些检测方法的相对优点。组织化学方法被认为对处理少量活检样本的实验室最为实用，但乙酰胆碱酯酶活性的生化测定被发现是一种有用的补充方法，并且能对酶活性进行准确的定量评估。
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Histochemical diagnosis of Hirschsprung's disease and a comparison of the histochemical and biochemical activity of acetylcholinesterase in rectal mucosal biopsies.先天性巨结肠的组织化学诊断以及直肠黏膜活检中乙酰胆碱酯酶组织化学与生化活性的比较
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