需要特定的磷脂来重构从大鼠脑中溶解出来的腺苷酸环化酶。
Specific phospholipids are required to reconstitute adenylate cyclase solubilized from rat brain.
作者信息
Hebdon G M, LeVine H, Sahyoun N E, Schmitges C J, Cuatrecasas P
出版信息
Proc Natl Acad Sci U S A. 1981 Jan;78(1):120-3. doi: 10.1073/pnas.78.1.120.
Adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] was solubilized from a rat brain homogenate with sodium deoxycholate. This solubilized preparation had no detectable enzymic activity with either Mg-ATP or Mn-ATP as substrate. The activity could be restored by addition of either nonionic detergent or certain specific phospholipids. Maximal restoration of enzyme activity was obtained with Triton X-100, L-alpha-phosphatidylcholine, L-alpha-lysophosphatidylcholine, phosphatidyl-N-monomethylethanolamine, or sphingomyelin. Activity was only partially restored by phosphatidylethanolamine (40-60%) or phosphatidyl-N,N-dimethylethanolamine (10-20%). Other phospholipids tested, including phosphatidylserine, phosphatidylglycerol, phosphatidylinositol, and phosphatidic acid, could not restore enzyme activity but, instead, could inhibit the stimulation of enzyme activity by phosphatidylcholine. The restoration of activity by L-alpha-phosphatidylcholine was inhibited by cholesterol at concentrations above 33 mol %, although this effect was not observed with three different esters of cholesterol. These studies suggest a possible specific role of phospholipids in modulating adenylate cyclase activity.
用脱氧胆酸钠从大鼠脑匀浆中溶解腺苷酸环化酶[ATP焦磷酸裂解酶（环化），EC 4.6.1.1]。这种溶解的制剂以Mg-ATP或Mn-ATP为底物时没有可检测到的酶活性。通过添加非离子洗涤剂或某些特定的磷脂可以恢复活性。用 Triton X-100、L-α-磷脂酰胆碱、L-α-溶血磷脂酰胆碱、磷脂酰-N-单甲基乙醇胺或鞘磷脂可获得酶活性的最大恢复。磷脂酰乙醇胺（40-60%）或磷脂酰-N,N-二甲基乙醇胺（10-20%）只能部分恢复活性。测试的其他磷脂，包括磷脂酰丝氨酸、磷脂酰甘油、磷脂酰肌醇和磷脂酸，不能恢复酶活性，反而会抑制磷脂酰胆碱对酶活性的刺激。当胆固醇浓度高于33 mol%时，L-α-磷脂酰胆碱对活性的恢复有抑制作用，不过三种不同的胆固醇酯未观察到这种效应。这些研究表明磷脂在调节腺苷酸环化酶活性方面可能具有特定作用。
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