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R6K复制终点周围的核苷酸序列。

The nucleotide sequence surrounding the replication terminus of R6K.

作者信息

Bastia D, Germino J, Crosa J H, Ram J

出版信息

Proc Natl Acad Sci U S A. 1981 Apr;78(4):2095-9. doi: 10.1073/pnas.78.4.2095.

DOI:10.1073/pnas.78.4.2095
PMID:6941271
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC319290/
Abstract

The replication terminus of the plasmid R6K has been cloned into the single-stranded DNA phage vector M13mp5 and also into the plasmid vectors pBR313 and PBR322. By using single-stranded DNA templates prepared from the recombinant DNA clones, the sequence of 215 base pairs of DNA containing the replication terminus has been determined. The DNA sequence of the region of the terminus does not contain any 2-fold rotational symmetry. Therefore, folding of the DNA at the region of the terminus is unlikely to be a cause for replication termination. Interaction of a host-specified protein(s) with the sequence of the replication terminus is probably the basis of the mechanism of replicaion termination.

摘要

质粒R6K的复制终止区已被克隆到单链DNA噬菌体载体M13mp5中，也被克隆到质粒载体pBR313和pBR322中。通过使用从重组DNA克隆体制备的单链DNA模板，已确定了包含复制终止区的215个碱基对的DNA序列。终止区区域的DNA序列不包含任何二重旋转对称。因此，DNA在终止区区域的折叠不太可能是复制终止的原因。宿主特异性蛋白质与复制终止区序列的相互作用可能是复制终止机制的基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d0/319290/9cbf759ed372/pnas00655-0141-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d0/319290/9cbf759ed372/pnas00655-0141-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d0/319290/9cbf759ed372/pnas00655-0141-a.jpg

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本文引用的文献

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Enzymatic breakage and joining of deoxyribonucleic acid. VI. Further purification and properties of polynucleotide ligase from Escherichia coli infected with bacteriophage T4.脱氧核糖核酸的酶促断裂与连接。VI. 来自感染噬菌体T4的大肠杆菌的多核苷酸连接酶的进一步纯化及性质
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Gene 6 exonuclease of bacteriophage T7. I. Purification and properties of the enzyme.
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The relationship between sequence-specific termination of DNA replication and transcription.DNA复制的序列特异性终止与转录之间的关系。
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