Ebert P S, Bonkowsky H L, Wars I
Chem Biol Interact. 1981 Jul;36(1):61-9. doi: 10.1016/0009-2797(81)90029-6.
The effects of short chain (C1-C5) aldehydes, ketones, acids alcohols and ethers on murine erythroleukemia (MEL) cells were examined to determine which particular chemical moieties and some of their combinations stimulated hemoglobin synthesis in these cells. The C4 series of compounds was active at lower concentrations than homologs of shorter chain lengths. Within an homologous series the potency and efficacy of the alcohol was always less then that of the acid and aldehyde compounds. Though hepanoic acid was found to be an inducer of hemoglobin synthesis in MEL cells, the 4,6-dioxoheptanoic acid analog is a potent inhibitor of hemoglobin synthesis. Analysis of porphyrin content of MEL cells incubated with the inducers 2-butanone, 2-methoxyethanol, acetone and methanol, showed that increased hemoglobin synthesis was always accompanied by the accumulation of porphyrins, most of which was protoporphyrin. These studies suggest that low molecular ketones, aldehydes, acids, ethers and alcohol can correct the defect in erythroid differentiation exhibited by MEL cells and they further suggest that the physiological trigger for inducing hemoglobin synthesis in these cells is less discriminating than previously recognized.
研究了短链（C1 - C5）醛、酮、酸、醇和醚对小鼠红白血病（MEL）细胞的影响，以确定哪些特定的化学基团及其某些组合能刺激这些细胞中的血红蛋白合成。C4系列化合物在比短链同系物更低的浓度下具有活性。在同系物系列中，醇的效力和效果总是低于酸和醛类化合物。虽然已发现庚酸是MEL细胞中血红蛋白合成的诱导剂，但4,6 - 二氧庚酸类似物是血红蛋白合成的有效抑制剂。对用诱导剂2 - 丁酮、2 - 甲氧基乙醇、丙酮和甲醇孵育的MEL细胞的卟啉含量分析表明，血红蛋白合成增加总是伴随着卟啉的积累，其中大部分是原卟啉。这些研究表明，低分子酮、醛、酸、醚和醇可以纠正MEL细胞所表现出的红系分化缺陷，并且进一步表明，诱导这些细胞中血红蛋白合成的生理触发因素比之前认为的更具普遍性。
