Kakpakova E S, Massino Iu S, Malalhova E M
Genetika. 1981;17(3):460-8.
Djungarian hamster cell lines resistant to actinomycin D (AD) were developed from SV40 transformed HGPRT- cell, line DM-15. Increase in resistance to AD up to 4000 fold was obtained. The acquisition of resistance to AD did not influence the expression of the first mutation--HGPRT-. The cells retained resistance to 6-mercaptopurine and could not grow in HAT medium, as well as the parent cell line DM-15. The acquisition of resistance to AD resulted in production of cell cultures with a less malignant phenotype, than that of the parent cell line DM-15. So, the cells resistant to AD had lower tumorigenicity in vivo, the reduced ability to form colonies in soft agar and were less transformed, as shown by morphological criteria. The obtained cell lines with two genetic markers--resistance to 2 microgram/ml of AD and HGPRT- can be used in somatic cell genetics, especially, for somatic hybridisation, and also to study the role of the cell membrane in malignant transformation.
抗放线菌素D（AD）的中国仓鼠细胞系是从经SV40转化的HGPRT-细胞系DM-15培育而来。对AD的抗性增加了4000倍。对AD抗性的获得并不影响第一个突变——HGPRT-的表达。这些细胞仍对6-巯基嘌呤有抗性，并且不能在HAT培养基中生长，这与亲本细胞系DM-15一样。对AD抗性的获得导致产生了一种细胞培养物，其恶性表型比亲本细胞系DM-15的要弱。因此，对AD有抗性的细胞在体内的致瘤性较低，在软琼脂中形成集落的能力降低，并且从形态学标准来看，其转化程度较低。所获得的具有两种遗传标记——对2微克/毫升AD有抗性和HGPRT-的细胞系可用于体细胞遗传学，特别是用于体细胞杂交，也可用于研究细胞膜在恶性转化中的作用。
