Malik Z, Djaldetti M
Int J Cancer. 1980 Oct 15;26(4):495-500. doi: 10.1002/ijc.2910260415.
The effect of protoporphyrin on erythroid, myeloid and lymphoid leukemic cells and their destruction induced by the photoactivated porphyrin was studied. Friend erythroleukemic cells (FL) and myelocytic leukemic cells (ML) accumulated protoporphyrin in a cap or patch-like pattern observed by fluorescence microscopy. Photoactivated protoporphyrin induced the appearance of "holes" on the cell membrane demonstrated by scanning electron microscopy. On the other hand, Burkitt lymphoma (BL) and mastocytoma (MS) cells accumulated porphyrin intracellularly around the nuclear envelope and as circular profiles, respectively. Photoactivated protoporphyrin induced development of multiple blebs on the cell membrane, and even complete cell destruction. Cytotoxicity of protoporphyrin at short-term incubation periods was determined by [3H]thymidine and [3H]uridine incorporation. Protoporphyrin, unexposed to light, reduced the incorporation of both precursors only to a moderate extent. On the other hand, porphyrin-treated cells exposed to light showed complete inhibition of RNA and DNA synthesis. Long-term exposure of ML and BL cells to porphyrin in the dark induced a nearly 50% inhibition of RNA and DNA synthesis. Although the cytotoxic effect of protoporphyrin in the dark was lower than that of photoactivated porphyrin, this may possess a potential activity in vivo even without illumination.
研究了原卟啉对红系、髓系和淋巴系白血病细胞的作用以及光活化卟啉对这些细胞的破坏作用。通过荧光显微镜观察发现，Friend红白血病细胞（FL）和髓性白血病细胞（ML）以帽状或斑块状模式积累原卟啉。扫描电子显微镜显示，光活化原卟啉可诱导细胞膜上出现“孔洞”。另一方面，伯基特淋巴瘤（BL）细胞和肥大细胞瘤（MS）细胞分别在核膜周围和细胞内以圆形分布的形式积累卟啉。光活化原卟啉可诱导细胞膜上出现多个小泡，甚至导致细胞完全破坏。通过[3H]胸腺嘧啶核苷和[3H]尿苷掺入法测定了原卟啉在短期孵育期的细胞毒性。未暴露于光的原卟啉仅在一定程度上降低了两种前体的掺入。另一方面，经卟啉处理并暴露于光的细胞显示RNA和DNA合成完全受到抑制。将ML和BL细胞在黑暗中长时间暴露于卟啉可导致RNA和DNA合成受到近50%的抑制。尽管原卟啉在黑暗中的细胞毒性作用低于光活化卟啉，但即使在没有光照的情况下，它在体内也可能具有潜在活性。
