Ramstack J M, Zuckerman L, Mockros L F, Caprini J A, Vagher J P
Res Commun Chem Pathol Pharmacol. 1978 Aug;21(2):327-42.
The coagulability of heparinized blood is shown to be a function of platelet and red cell concentrations. Thrombelastrographic tests were conducted on celite-activated fabricated whole blood samples with heparin concentrations ranging from zero to 1.46 units per ml of plasma, hematocrits from zero to 50% and platelet counts from zero to 300,000/mm3. The thrombelastograph provides three useful parameters of coagulability in native and heparinized blood: clot time, rate of clot stiffening and final clot stiffness. Of these, only the clot time is determined with the common clotting tests, while the rate and final clot stiffness are measures of clot quality that are found to be particularly sensitive to thrombocytopenia. The test results indicate (i) increases of platelet concentration produce the expected decrease in clot time, increase in rate of stiffening, and increase in final stiffness; (ii) increases of the red cell concentration from zero to 20% hematocrit, produce nonlinear increases in clotability, while further increases beyond 20% hematocrit produce no additional effects if the heparin concentration is less than 1.2 units/ml. Quantative relations are derived between the thrombelastographic variables, heparin concentration, and platelet concentration for those samples with hematocrits greater than 20%. These data indicate further experiments for the study of possible interaction between cellular blood elements and heparin.
肝素化血液的凝固性表现为血小板和红细胞浓度的函数。对硅藻土激活的人造全血样本进行了血栓弹力图测试，血浆中肝素浓度范围为每毫升零至1.46单位，血细胞比容范围为零至50%，血小板计数范围为零至300,000/mm³。血栓弹力图提供了天然血液和肝素化血液凝固性的三个有用参数：凝血时间、血凝块硬化速率和最终血凝块硬度。其中，只有凝血时间通过常规凝血试验测定，而硬化速率和最终血凝块硬度是血凝块质量的指标，发现它们对血小板减少特别敏感。测试结果表明：（i）血小板浓度增加会使凝血时间按预期缩短，硬化速率增加，最终硬度增加；（ii）血细胞比容从零增加到20%时，凝固性呈非线性增加，而如果肝素浓度低于1.2单位/毫升，血细胞比容超过20%后进一步增加不会产生额外影响。对于血细胞比容大于20%的样本，得出了血栓弹力图变量、肝素浓度和血小板浓度之间的定量关系。这些数据表明需要进一步开展实验来研究血液细胞成分与肝素之间可能的相互作用。
