Wilcox D R, Prakash S
Genetics. 1980 Dec;96(4):927-38. doi: 10.1093/genetics/96.4.927.
Twenty-six D. pseudoobscura strains isogenic for xanthine dehydrogenase alleles from Mesa Verde, Colorado, were tested for differences in the biochemical properties of different allelic forms of xanthine dehydrogenase. No significant differences in binding affinity (Km) or substrate specificity of the enzyme were found. Significant variation among strains, in activity (Vmax) and among electromorphs, as well as among strains, in thermolability was found. For the few strains tested, the activity and thermolability differences were shown to co-segregate with the electrophoretic mobility of the variant allele.
对来自科罗拉多州梅萨维德的26个因黄嘌呤脱氢酶等位基因而等基因的拟暗果蝇品系,测试了黄嘌呤脱氢酶不同等位基因形式的生化特性差异。未发现该酶的结合亲和力(Km)或底物特异性有显著差异。发现品系之间在活性(Vmax)方面、不同电变体之间以及品系之间在热稳定性方面存在显著差异。对于所测试的少数品系,活性和热稳定性差异与变异等位基因的电泳迁移率共分离。