黑腹果蝇自然种群中黄嘌呤脱氢酶基因座的隐藏电泳变异。
Hidden electrophoretic variation at the xanthine dehydrogenase locus in a natural population of Drosophila melanogaster.
作者信息
Buchanan B A, Johnson D L
出版信息
Genetics. 1983 Jun;104(2):301-15. doi: 10.1093/genetics/104.2.301.
Abstract
Sixty-two isochromosomal lines of D. melanogaster were screened for cryptic electrophoretic variation at the xanthine dehydrogenase (XDH) locus. Sequential polyacrylamide vertical slab gel electrophoresis was performed using four electrophoretic criteria. A total of 15 classes of electromorphs were revealed. D. melanogaster appears to exhibit as much polymorphism at this locus as other extensively studied Drosophila species.--No evidence for loci on the X or second chromosomes which modified XDH mobility was found. Six of the electromorphs were mapped to the Xdh (ry) structural locus. Eight of the remaining nine classes exhibited mobility variation consistent with structural variation at the Xdh locus. The final class exhibited aberrant patterns and is under further study.
摘要
对62条黑腹果蝇的等染色体系进行了筛查，以检测黄嘌呤脱氢酶（XDH）位点的隐性电泳变异。使用四种电泳标准进行连续的聚丙烯酰胺垂直平板凝胶电泳。共揭示了15类电变体。黑腹果蝇在该位点似乎表现出与其他经过广泛研究的果蝇物种一样多的多态性。——未发现X染色体或第二染色体上存在改变XDH迁移率的基因座的证据。其中6种电变体被定位到Xdh（ry）结构基因座。其余9类中的8类表现出与Xdh基因座结构变异一致的迁移率变化。最后一类表现出异常模式，正在进一步研究中。
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