Coyne J A
Genetics. 1976 Nov;84(3):593-607. doi: 10.1093/genetics/84.3.593.
Acrylamide gel electrophoresis was performed on the enzyme xanthine dehydrogenase in sixty isochromosomal lines of Drosophila persimilis from three geographic populations. Sequential electrophoretic analysis using varied gel concentrations and buffers revealed twenty-three alleles in this species where only five had been described previously. These new electrophoretic techniques also detected a profound increase in divergence of gene frequencies at this locus between D. persimilis and its sibling species D. pseudoobscura. The implications of these results for questions of speciation and the maintenance of genetic variability are discussed.
对来自三个地理种群的60个拟果蝇同染色体系的黄嘌呤脱氢酶进行了丙烯酰胺凝胶电泳。使用不同凝胶浓度和缓冲液的连续电泳分析揭示了该物种中的23个等位基因,而之前仅描述了其中5个。这些新的电泳技术还检测到拟果蝇与其近缘物种伪暗果蝇在该基因座处基因频率的差异显著增加。讨论了这些结果对物种形成问题和遗传变异性维持的影响。