引力场增强生物膜对蔗糖的通透性：对蔗糖空间假说的实验性反驳。
Gravitational field enhances permeability of biological membranes to sucrose: an experimental refutation of sucrose-space hypothesis.
作者信息
Sitaramam V, Sarma M K
出版信息
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3441-5. doi: 10.1073/pnas.78.6.3441.
Abstract
Isotonic conditions for the integrity of subcellular organelles are shown to be remarkably influenced by the concentration of sucrose present during their isolation by centrifugation. Using the technique of enzyme osmometry, we show that the content of sucrose in synaptosomes reflects nearly total equilibration across the membrane during centrifugation, due to altered permeability of membranes. Presence of sucrose in the matrix space of mitochondria, as demonstrated by enzyme osmometry of matrix enzymes, indicates that the sucrose-space hypothesis is invalid.
摘要
等渗条件对亚细胞器完整性的影响显著受离心分离过程中蔗糖浓度的影响。使用酶渗透压测定技术，我们发现突触体中蔗糖的含量反映了离心过程中膜两侧几乎完全的平衡，这是由于膜通透性的改变。通过对线粒体基质酶的酶渗透压测定表明，线粒体基质空间中存在蔗糖，这表明蔗糖空间假说是无效的。
相似文献
1
Gravitational field enhances permeability of biological membranes to sucrose: an experimental refutation of sucrose-space hypothesis.引力场增强生物膜对蔗糖的通透性：对蔗糖空间假说的实验性反驳。
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3441-5. doi: 10.1073/pnas.78.6.3441.
2
Free mitochondria and synaptosomes from single rat forebrain. A comparison between two known subfractionation techniques.从单个大鼠前脑中分离游离线粒体和突触体。两种已知分级分离技术的比较。
J Neurochem. 1985 Aug;45(2):653-6. doi: 10.1111/j.1471-4159.1985.tb04040.x.
3
Does sucrose space exist?蔗糖间隙存在吗？
J Theor Biol. 1981 Jun 7;90(3):317-36. doi: 10.1016/0022-5193(81)90315-5.
4
Studies on the non-linear osmotic pressure-volume relationship in mitochondria and entry of sucrose into the matrix space during centrifugation.线粒体中非线性渗透压-体积关系以及离心过程中蔗糖进入基质空间的研究。
Biochim Biophys Acta. 1983 Feb 17;722(2):256-70. doi: 10.1016/0005-2728(83)90072-5.
5
Alanine aminotransferase and some other enzymes in different populations of free brain cortex mitochondria.游离脑皮质线粒体不同群体中的丙氨酸转氨酶及其他一些酶。
Physiol Bohemoslov. 1981;30(4):323-8.
6
Effects of isolation and incubation on the biochemical and morphological integrity of synaptosomes.分离和孵育对突触体生化及形态完整性的影响。
Brain Res. 1980 Dec 8;202(2):469-73. doi: 10.1016/0006-8993(80)90157-2.
7
The intramitochondrial volume measured using sucrose as an extramitochondrial marker overestimates the true matrix volume determined with mannitol.使用蔗糖作为线粒体外标记物测量的线粒体内体积高估了用甘露醇测定的真实基质体积。
Biochem J. 1983 Aug 15;214(2):387-93. doi: 10.1042/bj2140387.
8
Protein synthesis by synaptosomes from rat brain. Contribution by the intraterminal mitochondria.大鼠脑突触体的蛋白质合成。终末内线粒体的作用。
Biochem J. 1974 Jul;142(1):7-17. doi: 10.1042/bj1420007.
9
Stabilizing effect of sucrose on leakage of cytoplasm from rat brain synaptosomes in saline media.蔗糖对盐溶液介质中大鼠脑突触体细胞质渗漏的稳定作用。
J Neurochem. 1977 Jun;28(6):1403-5. doi: 10.1111/j.1471-4159.1977.tb12342.x.
10
Biochemical and morphological integrity of subcellular organelles from human postmortem brain.来自人类死后大脑的亚细胞器的生化和形态完整性。
Neurobiology. 1974;4(1):12-20.
引用本文的文献
1
Stimulation of mitochondrial pyruvate metabolism and citrulline synthesis by dexamethasone. Effect of isolation and incubation media.地塞米松对线粒体丙酮酸代谢和瓜氨酸合成的刺激作用。分离和孵育培养基的影响。
Biochem J. 1984 Sep 1;222(2):379-87. doi: 10.1042/bj2220379.
2
Rat liver mitochondria prepared in mannitol media demonstrate increased mitochondrial volumes compared with mitochondria prepared in sucrose media. Relationship to the effect of glucagon on mitochondrial function.与在蔗糖培养基中制备的线粒体相比，在甘露醇培养基中制备的大鼠肝脏线粒体显示出线粒体体积增加。与胰高血糖素对线粒体功能的影响的关系。
Biochem J. 1984 Jul 1;221(1):147-52. doi: 10.1042/bj2210147.
3
The reversible Ca2+-induced permeabilization of rat liver mitochondria.大鼠肝脏线粒体中可逆的钙离子诱导通透性转变
Biochem J. 1986 Oct 1;239(1):19-29. doi: 10.1042/bj2390019.
4
Hydrogen peroxide permeation across liposomal membranes: a novel method to assess structural flaws in liposomes.
Mol Cell Biochem. 1989;91(1-2):91-7. doi: 10.1007/BF00228083.
5
Measurements of mitochondrial volumes are affected by the amount of mitochondria used in the determinations.线粒体体积的测量受测定中所用线粒体数量的影响。
Biochem J. 1987 Jul 15;245(2):375-9. doi: 10.1042/bj2450375.
本文引用的文献
1
Spectrophotometric measurements of the enzymatic formation of fumaric and cis-aconitic acids.富马酸和顺乌头酸酶促形成的分光光度测量。
Biochim Biophys Acta. 1950 Jan;4(1-3):211-4. doi: 10.1016/0006-3002(50)90026-6.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
Water uptake and extrusion by mitochondria in relation to oxidative phosphorylation.线粒体的水摄取与排出及其与氧化磷酸化的关系
Physiol Rev. 1962 Jul;42:467-517. doi: 10.1152/physrev.1962.42.3.467.
4
Tissue fractionation studies. 3. Further observations on the binding of acid phosphatase by rat-liver particles.组织分级分离研究。3. 关于大鼠肝脏颗粒对酸性磷酸酶结合的进一步观察。
Biochem J. 1955 Mar;59(3):426-33. doi: 10.1042/bj0590426.
5
Cholinergic and non-cholinergic nerve endings in rat brain. I. Isolation and subcellular distribution of acetylcholine and acetylcholinesterase.大鼠脑中的胆碱能和非胆碱能神经末梢。I. 乙酰胆碱和乙酰胆碱酯酶的分离及亚细胞分布
J Neurochem. 1962 Jan-Feb;9:23-35. doi: 10.1111/j.1471-4159.1962.tb07489.x.
6
The study of steady-state concentrations of internal solutes of mitochondria by rapid centrifugal transfer to a fixation medium.通过快速离心转移至固定介质来研究线粒体内部溶质的稳态浓度。
Biochem J. 1957 May;66(1):79-91. doi: 10.1042/bj0660079.
7
Some permeability properties of isolated rat liver cell mitochondria.分离的大鼠肝细胞线粒体的一些通透性特性。
J Gen Physiol. 1956 Sep 20;40(1):47-71. doi: 10.1085/jgp.40.1.47.
8
Colorimetric estimation of ketopentoses and ketohexoses.戊酮糖和己酮糖的比色测定
Biochem J. 1956 Aug;63(4):542-8. doi: 10.1042/bj0630542.
9
The osmotic behavior and permeability to non-electrolytes of mitochondria.线粒体的渗透行为及对非电解质的通透性
Arch Biochem Biophys. 1955 Sep;58(1):52-67. doi: 10.1016/0003-9861(55)90092-8.
Zotero 插件